Cell Biology Antibodies 12

Anti-PLA2G6 Antibody (CAB9492)

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SKU:
CAB9492
Product Type:
Antibody
Applications:
WB
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PLA2G6 Antibody
Antibody SKU:CAB9492
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human PLA2G6 (NP_003551.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human PLA2G6 (NP_003551.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RGSR PYES GPLE EFLK REFG EHTK MTDV RKPK VMLT GTLS DRQP AELH LFRN YDAP ETVR EPRF NQNV NLRP PAQP SDQL VWRA ARSS GAAP TYFR PNGR F
Gene ID:8398
Uniprot:O60733
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:46kDa/53kDa/84kDa/89kDa
Observed MW:105kDa
Synonyms:PLA2G6, CaI-PLA2, GVI, INAD1, IPLA2-VIA, NBIA2, NBIA2A, NBIA2B, PARK14, PLA2, PNPLA9, iPLA2, iPLA2beta
Background:The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date.
UniProt Protein Function:PLA2G6: Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F- actin polymerization at the pseudopods. Defects in PLA2G6 are the cause of neurodegeneration with brain iron accumulation type 2B (NBIA2B). A neurodegenerative disorder associated with iron accumulation in the brain, primarily in the basal ganglia. It is characterized by progressive extrapyramidal dysfunction leading to rigidity, dystonia, dysarthria and sensorimotor impairment. Defects in PLA2G6 are the cause of neurodegeneration with brain iron accumulation type 2A (NBIA2A); also known as Seitelberger disease. NBIA2A is a neurodegenerative disease characterized by pathologic axonal swelling and spheroid bodies in the central nervous system. Onset is within the first 2 years of life with death by age 10 years. Defects in PLA2G6 are the cause of Parkinson disease type 14 (PARK14). An adult-onset progressive neurodegenerative disorder characterized by parkinsonism, dystonia, severe cognitive decline, cerebral and cerebellar atrophy and absent iron in the basal ganglia on magnetic resonance imaging. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - linoleic acid; EC 3.1.1.4; Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Phospholipase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; extracellular space; microtubule organizing center

Molecular Function:hydrolase activity; phospholipase A2 activity

Biological Process: antibacterial humoral response; cardiolipin biosynthetic process

Disease: Neurodegeneration With Brain Iron Accumulation 2a; Neurodegeneration With Brain Iron Accumulation 2b; Parkinson Disease 14, Autosomal Recessive

NCBI Summary:The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date. [provided by RefSeq, Dec 2010]
UniProt Code:O60733
NCBI GenInfo Identifier:6685712
NCBI Gene ID:8398
NCBI Accession:O60733.2
UniProt Secondary Accession:O60733,O75645, Q8N452, Q9UG29, Q9UIT0, Q9Y671, A8K597 B0QYE8,
UniProt Related Accession:O60733
Molecular Weight:46,484 Da
NCBI Full Name:85/88 kDa calcium-independent phospholipase A2
NCBI Synonym Full Names:phospholipase A2 group VI
NCBI Official Symbol:PLA2G6  
NCBI Official Synonym Symbols:GVI; PLA2; INAD1; NBIA2; iPLA2; NBIA2A; NBIA2B; PARK14; PNPLA9; CaI-PLA2; IPLA2-VIA; iPLA2beta  
NCBI Protein Information:85/88 kDa calcium-independent phospholipase A2
UniProt Protein Name:85/88 kDa calcium-independent phospholipase A2
UniProt Synonym Protein Names:Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9
Protein Family:85/88 kDa calcium-independent phospholipase
UniProt Gene Name:PLA2G6  
UniProt Entry Name:PLPL9_HUMAN
Anti-PLA2G6 Polyclonal Antibody (CAB9492)Western blot analysis of extracts of rat liver, using PLA2G6 antibody (CAB9492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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