Metabolism Antibodies 1
Anti-PLA2G4A Antibody (CAB0394)
- SKU:
- CAB0394
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
Antibody Name: | Anti-PLA2G4A Antibody |
Antibody SKU: | CAB0394 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB |
Reactivity: | Human, Mouse |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PLA2G4A (NP_077734.1). |
Application: | WB |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse |
Positive Samples: | THP-1, NCI-H460, HT-29, HepG2, Mouse liver, Mouse lung |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PLA2G4A (NP_077734.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | MSFI DPYQ HIIV EHQY SHKF TVVV LRAT KVTK GAFG DMLD TPDP YVEL FIST TPDS RKRT RHFN NDIN PVWN ETFE FILD PNQE NVLE ITLM DANY VMDE TLGT ATFT VSSM KVGE KKEV PFIF NQVT EMVL EMSL EVCS CPDL RFSM ALCD QEKT FRQQ RKEH IRES MKKL LGPK NSEG |
Gene ID: | 5321 |
Uniprot: | P47712 |
Cellular Location: | Cytoplasm, Cytoplasmic vesicle |
Calculated MW: | 85kDa |
Observed MW: | 115kDa |
Synonyms: | PLA2G4A, PLA2G4, cPLA2, cPLA2-alpha |
Background: | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. |
UniProt Protein Function: | cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A. |
UniProt Protein Details: | Protein type:Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Phospholipase; EC 3.1.1.5; Lipid Metabolism - arachidonic acid Chromosomal Location of Human Ortholog: 1q25 Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol Molecular Function:phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity Biological Process: icosanoid biosynthetic process; platelet activating factor biosynthetic process; platelet activation; phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; icosanoid metabolic process; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation |
NCBI Summary: | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008] |
UniProt Code: | P47712 |
NCBI GenInfo Identifier: | 317373312 |
NCBI Gene ID: | 5321 |
NCBI Accession: | P47712.2 |
UniProt Secondary Accession: | P47712,Q29R80, B1AKG4, |
UniProt Related Accession: | P47712 |
Molecular Weight: | 85,239 Da |
NCBI Full Name: | Cytosolic phospholipase A2 |
NCBI Synonym Full Names: | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
NCBI Official Symbol: | PLA2G4AÂ Â |
NCBI Official Synonym Symbols: | PLA2G4; cPLA2-alpha  |
NCBI Protein Information: | cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein |
UniProt Protein Name: | Cytosolic phospholipase A2 |
UniProt Synonym Protein Names: | Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase |
Protein Family: | Phospholipase |
UniProt Gene Name: | PLA2G4AÂ Â |
UniProt Entry Name: | PA24A_HUMAN |