Metabolism Antibodies 2
Anti-PLA2G1B Antibody (CAB5478)
- SKU:
- CAB5478
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PLA2G1B Antibody |
| Antibody SKU: | CAB5478 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-148 of human PLA2G1B (NP_000919.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, Mouse pancreas, Mouse small intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-148 of human PLA2G1B (NP_000919.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AVWQ FRKM IKCV IPGS DPFL EYNN YGCY CGLG GSGT PVDE LDKC CQTH DNCY DQAK KLDS CKFL LDNP YTHT YSYS CSGS AITC SSKN KECE AFIC NCDR NAAI CFSK APYN KAHK NLDT KKYC QS |
| Gene ID: | 5319 |
| Uniprot: | P04054 |
| Cellular Location: | Secreted |
| Calculated MW: | 16kDa |
| Observed MW: | 16kDa |
| Synonyms: | PLA2G1B, PLA2, PLA2A, PPLA2 |
| Background: | This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. |
| UniProt Protein Function: | PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Secreted; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; DNA replication; Lipid Metabolism - linoleic acid Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: extracellular space; cell surface; extracellular region; secretory granule Molecular Function:phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding Biological Process: positive regulation of immune response; activation of MAPK activity; neutrophil mediated immunity; activation of phospholipase A2; glucose transport; signal transduction; activation of NF-kappaB transcription factor; phosphatidylcholine metabolic process; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; neutrophil chemotaxis; multicellular organismal lipid catabolic process; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; cellular response to insulin stimulus; innate immune response in mucosa; positive regulation of protein secretion; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; interleukin-8 production; positive regulation of DNA replication |
| NCBI Summary: | This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P04054 |
| NCBI GenInfo Identifier: | 4505847 |
| NCBI Gene ID: | 5319 |
| NCBI Accession: | NP_000919.1 |
| UniProt Secondary Accession: | P04054,Q3KPI1, B2R4H5, |
| UniProt Related Accession: | P04054 |
| Molecular Weight: | 16,360 Da |
| NCBI Full Name: | phospholipase A2 |
| NCBI Synonym Full Names: | phospholipase A2, group IB (pancreas) |
| NCBI Official Symbol: | PLA2G1B |
| NCBI Official Synonym Symbols: | PLA2; PLA2A; PPLA2 |
| NCBI Protein Information: | phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B |
| UniProt Protein Name: | Phospholipase A2 |
| UniProt Synonym Protein Names: | Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B |
| Protein Family: | Phospholipase |
| UniProt Gene Name: | PLA2G1B |
| UniProt Entry Name: | PA21B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLA2G1B antibody (CAB5478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse pancreas using PLA2G1B antibody (CAB5478) at dilution of 1:100 (40x lens). |
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