Immunology Antibodies 2
Anti-PLA2G16 Antibody (CAB16018)
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- SKU:
- CAB16018
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-PLA2G16 Antibody |
| Antibody SKU: | CAB16018 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 68-162 of human PLA2G16 (NP_009000.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HepG2, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 68-162 of human PLA2G16 (NP_009000.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VAGS DKYQ VNNK HDDK YSPL PCSK IIQR AEEL VGQE VLYK LTSE NCEH FVNE LRYG VARS DQVR DVII AASV AGMG LAAM SLIG VMFS RNKR QKQ |
| Gene ID: | 11145 |
| Uniprot: | P53816 |
| Cellular Location: | |
| Calculated MW: | 18kDa |
| Observed MW: | 18kDa |
| Synonyms: | PLA2G16, AdPLA, H-REV107, H-REV107-1, HRASLS3, HREV107, HREV107-1, HREV107-3, HRSL3 |
| Background: |
| UniProt Protein Function: | PLA2G16: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. N- and O- acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Belongs to the H-rev107 family. |
| UniProt Protein Details: | Protein type:EC 3.1.1.4; EC 3.1.1.32; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q12.3 Cellular Component: perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; cytosol Molecular Function:phospholipase A2 activity; protein binding; phospholipase A1 activity Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; lipid catabolic process; negative regulation of cell cycle |
| UniProt Code: | P53816 |
| NCBI GenInfo Identifier: | 75517991 |
| NCBI Gene ID: | 11145 |
| NCBI Accession: | |
| UniProt Related Accession: | P53816,Q3MI98 |
| Molecular Weight: | 21,441 Da |
| NCBI Full Name: | PLA2G16 protein |
| NCBI Synonym Full Names: | phospholipase A2, group XVI |
| NCBI Official Symbol: | PLA2G16 |
| NCBI Official Synonym Symbols: | AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; MGC118754; H-REV107-1 |
| NCBI Protein Information: | group XVI phospholipase A2; OTTHUMP00000237069; adipose-specific PLA2; HRAS-like suppressor 1; HRAS-like suppressor 3; H-rev 107 protein homolog; Ca-independent phospholipase A1/2; adipose-specific phospholipase A2; renal carcinoma antigen NY-REN-65 |
| UniProt Protein Name: | PLA2G16 protein |
| Protein Family: | HRAS-like suppressor |
| UniProt Gene Name: | PLA2G16 |
| UniProt Entry Name: | Q3MI98_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLA2G16 antibody (CAB16018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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