Metabolism Antibodies 1

Anti-PLA2G10 Antibody (CAB10256)

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SKU:
CAB10256
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PLA2G10 Antibody
Antibody SKU:CAB10256
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PLA2G10 (NP_003552.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:K-562
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PLA2G10 (NP_003552.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TVGC VGPR TPIA YMKY GCFC GLGG HGQP RDAI DWCC HGHD CCYT RAEE AGCS PKTE RYSW QCVN QSVL CGPA ENKC QELL CKCD QEIA NCLA QTEY NLKY L
Gene ID:8399
Uniprot:O15496
Cellular Location:Secreted
Calculated MW:18kDa
Observed MW:18kDa
Synonyms:PLA2G10, GXPLA2, GXSPLA2, SPLA2, sPLA2-X
Background:This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism.
UniProt Protein Function:PLA2G10: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. Belongs to the phospholipase A2 family.
UniProt Protein Details:

Protein type:Cell development/differentiation; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis; Phospholipase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.12

Cellular Component: extracellular region

Molecular Function:phospholipase A2 activity; phospholipase activity

Biological Process: axon guidance; cholesterol homeostasis; lysophospholipid transport; negative regulation of transcription factor activity; phosphatidic acid biosynthetic process; positive regulation of cellular protein metabolic process; positive regulation of prostaglandin secretion; regulation of macrophage activation

NCBI Summary:This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism. [provided by RefSeq, Nov 2015]
UniProt Code:O15496
NCBI GenInfo Identifier:239938869
NCBI Gene ID:8399
NCBI Accession:O15496.3
UniProt Secondary Accession:O15496,Q14DU3, Q6NT23,
UniProt Related Accession:O15496
Molecular Weight:18kDa
NCBI Full Name:Group 10 secretory phospholipase A2
NCBI Synonym Full Names:phospholipase A2 group X
NCBI Official Symbol:PLA2G10  
NCBI Official Synonym Symbols:SPLA2; GXPLA2; GXSPLA2; sPLA2-X  
NCBI Protein Information:group 10 secretory phospholipase A2
UniProt Protein Name:Group 10 secretory phospholipase A2
UniProt Synonym Protein Names:Group X secretory phospholipase A2; GX sPLA2; sPLA2-X; Phosphatidylcholine 2-acylhydrolase 10
Protein Family:Group 10 secretory phospholipase
UniProt Gene Name:PLA2G10  
Anti-PLA2G10 Antibody (CAB10256)Western blot analysis of extracts of K562 cells, using PLA2G10 antibody (CAB10256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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