Name: Anti-PKP2 Antibody (CAB5507)
Brand: Cell Biology Antibodies 9
SKU: CAB5507
Availability: OutOfStock

Antibody Name:	Anti-PKP2 Antibody
Antibody SKU:	CAB5507
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 578-837 of human PKP2 (NP_001005242.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, Mouse kidney, Mouse liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 578-837 of human PKP2 (NP_001005242.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LPEK YSQN IYIQ NRNI QTDN NKSI GCFG SRSR KVKE QYQD VPMP EEKS NPKG VEWL WHSI VIRM YLSL IAKS VRNY TQEA SLGA LQNL TAGS GPMP TSVA QTVV QKES GLQH TRKM LHVG DPSV KKTA ISLL RNLS RNLS LQNE IAKE TLPD LVSI IPDT VPST DLLI ETTA SACY TLNN IIQN SYQN ARDL LNTG GIQK IMAI SAGD AYAS NKAS KAAS VLLY SLWA HTEL HHAY KKAQ FKKT DFVN SRTA KAYH SLKD
Gene ID:	5318
Uniprot:	Q99959
Cellular Location:	Cell junction, Nucleus, desmosome
Calculated MW:	92kDa/97kDa
Observed MW:	95-105kDa

Synonyms:

PKP2, ARVD9

Background:

This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13.

UniProt Protein Function:	plakophilin 2: May play a role in junctional plaques. Defects in PKP2 are the cause of familial arrhythmogenic right ventricular dysplasia type 9 (ARVD9); also known as arrhythmogenic right ventricular cardiomyopathy 9 (ARVC9). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Belongs to the beta-catenin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 12p11 Cellular Component: nucleoplasm; desmosome; adherens junction; plasma membrane; integral to membrane; intermediate filament; intercellular junction; nucleus; cell junction Molecular Function:protein binding; sodium channel regulator activity; protein kinase C binding; intermediate filament binding; protein complex scaffold Biological Process: negative regulation of cell proliferation; cell-cell adhesion; gap junction assembly; heart development; lipid homeostasis; ventricular cardiac muscle morphogenesis; intermediate filament bundle assembly; maintenance of organ identity; negative regulation of cell migration Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 9
NCBI Summary:	This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99959
NCBI GenInfo Identifier:	296452867
NCBI Gene ID:	5318
NCBI Accession:	Q99959.2
UniProt Secondary Accession:	Q99959,Q4VC01, Q99960, A0AV37, B8QFA1, B8QGS6, B8QGS7 D3DUW9,
UniProt Related Accession:	Q99959
Molecular Weight:	92,756 Da
NCBI Full Name:	Plakophilin-2
NCBI Synonym Full Names:	plakophilin 2
NCBI Official Symbol:	PKP2
NCBI Official Synonym Symbols:	ARVD9
NCBI Protein Information:	plakophilin-2
UniProt Protein Name:	Plakophilin-2
Protein Family:	Plakophilin
UniProt Gene Name:	PKP2
UniProt Entry Name:	PKP2_HUMAN

Western blot analysis of extracts of various cell lines, using PKP2 antibody (CAB5507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

PKP2 Antibody (PACO06766)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)