Anti-PKM2-specific Antibody (CAB20991)
- SKU:
- CAB20991
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-PKM2-specific Antibody (CAB20991) |
| Antibody SKU: | CAB20991 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunoprecipitation |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PKM2 (XP_006720633.1). |
| Application: | Western blotting, Immunoprecipitation |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, NIH/3T3, C6 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PKM2 (XP_006720633.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RMQH LIAR EAEA AIYH LQLF EELR RLAP ITSD PTEA TAVG AVEA SFKC CSGA IIVL TKSG RSAH QVAR YRPR APII AVTR NPQT ARQA HLYR GIFP VLCK D |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 58kDa |
| Observed MW: | 60kDa |
| Synonyms: | CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, PKM |
| Background: | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. provided by RefSeq, May 2011 |
 | Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug PKM2-specific antibody . Western blot was performed from the immunoprecipitate using PKM2-specific antibody at a dilition of 1:1000. |
 | Western blot analysis of extracts of various cell lines, using PKM2-specific antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. |
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