Metabolism Antibodies 1

Anti-PKM Antibody (CAB13905)

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SKU:
CAB13905
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PKM Antibody
Antibody SKU:CAB13905
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM (NP_002645.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, A-549, Mouse skeletal muscle, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM (NP_002645.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AFIQ TQQL HAAM ADTF LEHM CRLD IDSP PITA RNTG IICT IGPA SRSV ETLK EMIK SGMN VARL NFSH GTHE YHAE TIKN VRTA TESF ASDP ILYR PVAV ALDT KGPE IRTG LIKG SGTA EVEL KKGA TLKI TLDN AYME KCDE NILW LDYK NICK VVEV GSKI YVDD GLIS LQVK QKGA DFLV TEVE NGGS LGSK KGVN LPGA AVDL PAV
Gene ID:5315
Uniprot:P14618
Cellular Location:Cytoplasm, Nucleus
Calculated MW:56kDa/57kDa/58kDa
Observed MW:60KDa
Synonyms:CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, PKM
Background:This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
UniProt Protein Function:PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Mitochondrial; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium

Molecular Function:potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation

NCBI Summary:This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Code:P14618
NCBI GenInfo Identifier:20178296
NCBI Gene ID:5315
NCBI Accession:P14618.4
UniProt Secondary Accession:P14618,P14786, Q53GK4, Q96E76, Q9BWB5, Q9UCV6, Q9UPF2 A6NFK3, B2R5N8, B3KRY0, B4DFX8, B4DUU6,
UniProt Related Accession:P14618
Molecular Weight:
NCBI Full Name:Pyruvate kinase PKM
NCBI Synonym Full Names:pyruvate kinase, muscle
NCBI Official Symbol:PKM  
NCBI Official Synonym Symbols:PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30  
NCBI Protein Information:pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic
UniProt Protein Name:Pyruvate kinase PKM
UniProt Synonym Protein Names:Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
Protein Family:Pyruvate kinase
UniProt Gene Name:PKM  
UniProt Entry Name:KPYM_HUMAN
Anti-PKM Antibody (CAB13905)Western blot analysis of extracts of various cell lines, using PKM antibody (CAB13905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-PKM Antibody (CAB13905)Immunohistochemistry of paraffin-embedded Mouse kidney using PKM Rabbit pAb (CAB13905) at dilution of 1:100 (40x lens).
Anti-PKM Antibody (CAB13905)Immunohistochemistry of paraffin-embedded Rat lung using PKM Rabbit pAb (CAB13905) at dilution of 1:100 (40x lens).
Anti-PKM Antibody (CAB13905)Immunohistochemistry of paraffin-embedded Human colon using PKM Rabbit pAb (CAB13905) at dilution of 1:100 (40x lens).
Anti-PKM Antibody (CAB13905)Immunofluorescence analysis of L929 cells using PKM Rabbit pAb (CAB13905) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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