Cell Biology Antibodies 8
Anti-PKD2 Antibody (CAB3625)
- SKU:
- CAB3625
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PKD2 Antibody |
| Antibody SKU: | CAB3625 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PKD2 (NP_000288.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, MCF7, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PKD2 (NP_000288.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GMMS SNVY YYTR MMSQ LFLD TPVS KTEK TNFK TLSS MEDF WKFT EGSL LDGL YWKM QPSN QTEA DNRS FIFY ENLL LGVP RIRQ LRVR NGSC SIPQ DLRD EIKE CYDV YSVS SEDR APFG PRNG TAWI YTSE KDLN GSSH WGII ATYS GAGY YLDL SRTR EETA AQVA SLK |
| Gene ID: | 5311 |
| Uniprot: | Q13563 |
| Cellular Location: | Cell projection, Endoplasmic reticulum, Multi-pass membrane protein, cilium membrane |
| Calculated MW: | 53kDa/73kDa/98kDa/103kDa/109kDa |
| Observed MW: | 110kDa |
| Synonyms: | PKD2, APKD2, PC2, PKD4, Pc-2, TRPP2 |
| Background: | This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. |
| UniProt Protein Function: | PKD2: Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Functions as a calcium permeable cation channel. Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2). ADPKD2 is a disorder characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. It represents approximately 15% of the cases of autosomal dominant polycystic kidney disease. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy. Belongs to the polycystin family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Channel, cation; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: filamentous actin; endoplasmic reticulum membrane; nonmotile primary cilium; endoplasmic reticulum; integral to plasma membrane; lamellipodium; cytoplasm; basal plasma membrane; plasma membrane; motile primary cilium; intercellular junction; basal cortex; cilium Molecular Function:voltage-gated cation channel activity; actinin binding; identical protein binding; protein homodimerization activity; phosphoprotein binding; voltage-gated ion channel activity; calcium ion binding; muscle alpha-actinin binding; ATPase binding; calcium-induced calcium release activity; voltage-gated calcium channel activity; protein binding; potassium channel activity; voltage-gated sodium channel activity; cytoskeletal protein binding; HLH domain binding; receptor binding Biological Process: neural tube development; positive regulation of inositol-1,4,5-triphosphate receptor activity; embryonic placenta development; cytoplasmic sequestering of transcription factor; positive regulation of nitric oxide biosynthetic process; regulation of cAMP metabolic process; heart development; detection of mechanical stimulus; JAK-STAT cascade; liver development; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; regulation of cell proliferation; negative regulation of cell proliferation; ureteric bud branching; spinal cord development; release of sequestered calcium ion into cytosol; calcium ion transport; positive regulation of transcription from RNA polymerase II promoter; heart looping; cell cycle arrest; determination of left/right symmetry; centrosome duplication Disease: Polycystic Kidney Disease 2 |
| NCBI Summary: | This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q13563 |
| NCBI GenInfo Identifier: | 116242717 |
| NCBI Gene ID: | 5311 |
| NCBI Accession: | Q13563.3 |
| UniProt Secondary Accession: | Q13563,O60441, Q15764, Q2M1Q3, Q2M1Q5, |
| UniProt Related Accession: | Q13563 |
| Molecular Weight: | 968 |
| NCBI Full Name: | Polycystin-2 |
| NCBI Synonym Full Names: | polycystic kidney disease 2 (autosomal dominant) |
| NCBI Official Symbol: | PKD2 |
| NCBI Official Synonym Symbols: | PC2; PKD4; Pc-2; APKD2; TRPP2 |
| NCBI Protein Information: | polycystin-2; R48321; polycystwin; autosomal dominant polycystic kidney disease type II protein; transient receptor potential cation channel, subfamily P, member 2 |
| UniProt Protein Name: | Polycystin-2 |
| UniProt Synonym Protein Names: | Autosomal dominant polycystic kidney disease type II protein; Polycystic kidney disease 2 protein; Polycystwin; R48321 |
| Protein Family: | Polycystin |
| UniProt Gene Name: | PKD2 |
| UniProt Entry Name: | PKD2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PKD2 antibody (CAB3625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
