Signal Transduction Antibodies 1
Anti-PITPNA Antibody (CAB12966)
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- SKU:
- CAB12966
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-PITPNA Antibody |
| Antibody SKU: | CAB12966 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 211-270 of human PITPNA (NP_006215.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 211-270 of human PITPNA (NP_006215.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NFIH KQER RLFT NFHR QLFC WLDK WVDL TMDD IRRM EEET KRQL DEMR QKDP VKGM TADD |
| Gene ID: | 5306 |
| Uniprot: | Q00169 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 31kDa |
| Observed MW: | 35kDa |
| Synonyms: | PITPNA, HEL-S-36, PI-TPalpha, PITPN, VIB1A |
| Background: | This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) by phosphoinositide-3-kinase. |
| UniProt Protein Function: | PITPNA: Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes. Belongs to the PtdIns transfer protein family. PI transfer class I subfamily. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 17p13.3 Molecular Function:phosphatidylcholine transmembrane transporter activity; phosphatidylinositol transporter activity Biological Process: visual perception |
| NCBI Summary: | This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) by phosphoinositide-3-kinase.[provided by RefSeq, Sep 2009] |
| UniProt Code: | Q00169 |
| NCBI GenInfo Identifier: | 130770 |
| NCBI Gene ID: | 5306 |
| NCBI Accession: | Q00169.2 |
| UniProt Secondary Accession: | Q00169,P16446, P48738, P53810, Q2HJ54, |
| UniProt Related Accession: | Q00169 |
| Molecular Weight: | 31,806 Da |
| NCBI Full Name: | Phosphatidylinositol transfer protein alpha isoform |
| NCBI Synonym Full Names: | phosphatidylinositol transfer protein alpha |
| NCBI Official Symbol: | PITPNA |
| NCBI Official Synonym Symbols: | PITPN; VIB1A; HEL-S-36; PI-TPalpha |
| NCBI Protein Information: | phosphatidylinositol transfer protein alpha isoform |
| UniProt Protein Name: | Phosphatidylinositol transfer protein alpha isoform |
| Protein Family: | Phosphatidylinositol transfer protein |
| UniProt Gene Name: | PITPNA |
| UniProt Entry Name: | PIPNA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PITPNA antibody (CAB12966) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of L929 cells using PITPNA Rabbit pAb (CAB12966) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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