Cell Biology Antibodies 11
Anti-PIP5K1B Antibody (CAB7749)
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- SKU:
- CAB7749
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PIP5K1B Antibody |
| Antibody SKU: | CAB7749 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-470 of human PIP5K1B (NP_003549.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-470 of human PIP5K1B (NP_003549.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NVLP RSMR MHFT YDLK GSTY KRRA SRKE REKS NPTF KDLD FLQD MHEG LYFD TETY NALM KTLQ RDCR VLES FKIM DYSL LLGI HFLD HSLK EKEE ETPQ NVPD AKRT GMQK VLYS TAME SIQG PGKS GDGI ITEN PDTM GGIP AKSH RGEK LLLF MGII DILQ SYRL MKKL EHSW KALV YDGD TVSV HRPS FYAD RFLK FMNS RVFK KIQA LKAS PSKK RCNS IAAL KATS QEIV SSIS QEWK DEKR DLLT EGQS FSSL DEEA LGSR HRPD LVPS TPSL F |
| Gene ID: | 8395 |
| Uniprot: | O14986 |
| Cellular Location: | Endomembrane system |
| Calculated MW: | 56kDa/61kDa/62kDa |
| Observed MW: | 71kDa |
| Synonyms: | PIP5K1B, MSS4, STM7 |
| Background: |
| UniProt Protein Function: | PIP5K1B: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68; Kinase, lipid Chromosomal Location of Human Ortholog: 9q13 Cellular Component: membrane; endomembrane system; uropod; cytosol Molecular Function:protein binding; 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process |
| UniProt Code: | O14986 |
| NCBI GenInfo Identifier: | 78099085 |
| NCBI Gene ID: | 8395 |
| NCBI Accession: | O14986.2 |
| UniProt Secondary Accession: | O14986,P78518, P78519, Q5T5K6, Q5T5K8, Q5T5K9, Q5VZ00 Q7KYT5, Q8NHQ5, Q92749, A8K9L9, B4DIG7, |
| UniProt Related Accession: | O14986 |
| Molecular Weight: | 540 |
| NCBI Full Name: | Phosphatidylinositol 4-phosphate 5-kinase type-1 beta |
| NCBI Synonym Full Names: | phosphatidylinositol-4-phosphate 5-kinase, type I, beta |
| NCBI Official Symbol: | PIP5K1B |
| NCBI Official Synonym Symbols: | MSS4; STM7 |
| NCBI Protein Information: | phosphatidylinositol 4-phosphate 5-kinase type-1 beta; PIP5KIbeta; PIP5K1-beta; protein STM-7; ptdIns(4)P-5-kinase 1 beta; phosphatidylinositol 4-phosphate 5-kinase type I beta; phosphatidylinositol-4-phosphate 5-kinase type-1 beta; type I phosphatidylinositol 4-phosphate 5-kinase beta; type I phosphatidylinositol-4-phosphate 5-kinase beta |
| UniProt Protein Name: | Phosphatidylinositol 4-phosphate 5-kinase type-1 beta |
| UniProt Synonym Protein Names: | Phosphatidylinositol 4-phosphate 5-kinase type I beta; PIP5KIbeta; Protein STM-7; Type I phosphatidylinositol 4-phosphate 5-kinase beta |
| Protein Family: | Phosphatidylinositol 4-phosphate 5-kinase |
| UniProt Gene Name: | PIP5K1B |
| UniProt Entry Name: | PI51B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PIP5K1B antibody (CAB7749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using PIP5K1B antibody (CAB7749) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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