Name: Anti-PIP5K1A Antibody (CAB18025)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18025
Availability: OutOfStock

Antibody Name:	Anti-PIP5K1A Antibody [KO Validated]
Antibody SKU:	CAB18025
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNNL LPRS VKMH IKYD LKGS TYKR RASQ KERE KPLP TFKD LDFL QDIP DGLF LDAD MYNA LCKT LQRD CLVL QSFK IMDY SLLM SIHN IDHA QREP LSSE TQYS VDTR RPAP QKAL YSTA MESI QGEA RRGG TMET DDHM GGIP ARNS KGER LLLY IGII DILQ SYRF VKKL EHSW KALV HDGD TVSV HRPG FYAE RFQR FMCN TVFK KIPC VHLG RPDV LPQT PPLE EISE GSPI PDPS FSPL VGET LQML TTST TLEK LEVA ESEF TH
Gene ID:	8394
Uniprot:	Q99755
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Nucleus speckle, ruffle
Calculated MW:	56kDa/58kDa/61kDa/62kDa
Observed MW:	63kDa

Anti-PIP5K1A Antibody [KO Validated] (CAB18025)

Western blot analysis of extracts from normal (control) and PIP5K1A knockout (KO) 293T cells, using PIP5K1A antibody (CAB18025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Synonyms:	PIP5K1A
Background:

UniProt Protein Function:	PIP5K1A: Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Interacts with RAC1. Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation. Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; EC 2.7.1.68; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: cytoplasm; cytosol; focal adhesion; lamellipodium; mRNA cleavage and polyadenylation specificity factor complex; nuclear speck; nucleoplasm; nucleus; plasma membrane Molecular Function:1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; kinase binding; protein binding Biological Process: actin cytoskeleton reorganization; focal adhesion formation; glycerophospholipid metabolic process; keratinocyte differentiation; phagocytosis; phosphatidylinositol biosynthetic process; phospholipid biosynthetic process; regulation of phosphoinositide 3-kinase cascade; signal transduction
UniProt Code:	Q99755
NCBI GenInfo Identifier:	74752158
NCBI Gene ID:	8394
NCBI Accession:	Q99755.1
UniProt Secondary Accession:	Q99755,Q99754, Q99756, A8K4Q0, B4DIN0,
UniProt Related Accession:	Q99755
Molecular Weight:	58,119 Da
NCBI Full Name:	Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
NCBI Synonym Full Names:	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
NCBI Official Symbol:	PIP5K1A
NCBI Protein Information:	phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Protein Name:	Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Synonym Protein Names:	68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; PIP5KIalpha
Protein Family:	Phosphatidylinositol 4-phosphate 5-kinase
UniProt Gene Name:	PIP5K1A
UniProt Entry Name:	PI51A_HUMAN