Cell Cycle Antibodies 1

Anti-PIN1 Antibody (CAB13665)

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SKU:
CAB13665
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PIN1 Antibody
Antibody SKU:CAB13665
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human PIN1 (NP_006212.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:293T, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human PIN1 (NP_006212.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADE EKLP PGWE KRMS RSSG RVYY FNHI TNAS QWER PSGN SSSG GKNG QGEP ARVR CSHL LVKH SQSR RPSS WRQE KITR TKEE ALEL INGY IQKI KSGE EDFE SLAS QFSD CSSA KARG DLGA FSRG QMQK PFED ASFA LRTG EMSG PVFT DSGI HIIL RTE
Gene ID:5300
Uniprot:Q13526
Cellular Location:Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:18kDa
Observed MW:17kDa
Synonyms:PIN1, DOD, UBL5
Background:Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).
NCBI Summary:Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
UniProt Code:Q13526
NCBI GenInfo Identifier:3024406
NCBI Gene ID:5300
NCBI Accession:Q13526.1
UniProt Secondary Accession:Q13526,Q53X75, A8K4V9,
UniProt Related Accession:Q13526
Molecular Weight:
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
NCBI Synonym Full Names:peptidylprolyl cis/trans isomerase, NIMA-interacting 1
NCBI Official Symbol:PIN1  
NCBI Official Synonym Symbols:DOD; UBL5  
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
UniProt Synonym Protein Names:Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1
Protein Family:Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:PIN1  
UniProt Entry Name:PIN1_HUMAN
Anti-PIN1 Antibody (CAB13665)Western blot analysis of extracts of various cell lines, using PIN1 antibody (CAB13665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PIN1 Antibody (CAB13665)Immunofluorescence analysis of MCF-7 cells using PIN1 antibody (CAB13665). Blue: DAPI for nuclear staining.
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