Cell Biology Antibodies 6
Anti-PIK3R4 Antibody (CAB15828)
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- SKU:
- CAB15828
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PIK3R4 Antibody |
| Antibody SKU: | CAB15828 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PIK3R4 (NP_055417.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PIK3R4 (NP_055417.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGNQ LAGI APSQ ILSV ESYF SDIH DFEY DKSL GSTR FFKV ARAK HREG LVVV KVFA IQDP TLPL TSYK QELE ELKI RLNS AQNC LPFQ KASE KASE KAAM LFRQ YVRD NLYD RIST RPFL NNIE KRWI AFQI LTAV DQAH KSGV RHGD IKTE NVMV TSWN WVLL TDFA SFKP TYLP EDNP ADFN YFFD TSRR RTCY IAPE RFVD GGMF ATEL EYMR DPST PLVD LNSN QRTR GELK RAMD IFSA GCVI AELF TEGV PLFD LSQL LAYR NGHF FPEQ VLNK IEDH SIRE LVTQ MIHR EPDK RLEA EDYL KQQR GNAF PEIF YTFL QPYM AQ |
| Gene ID: | 30849 |
| Uniprot: | Q99570 |
| Cellular Location: | Cytoplasmic vesicle, Late endosome, Lipid-anchor, Membrane, autophagosome |
| Calculated MW: | 153kDa |
| Observed MW: | 153kDa |
| Synonyms: | PIK3R4, VPS15, p150 |
| Background: |
| UniProt Protein Function: | PIK3R4: Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, lipid; EC 2.7.11.1; Other group; VPS15 family Chromosomal Location of Human Ortholog: 3q22.1 Cellular Component: membrane; late endosome; nuclear pore; axoneme; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity Biological Process: macroautophagy; phospholipid metabolic process; late endosome to vacuole transport; toll-like receptor signaling pathway; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; protein targeting to vacuole; toll-like receptor 9 signaling pathway; peroxisome degradation; protein amino acid phosphorylation |
| UniProt Code: | Q99570 |
| NCBI GenInfo Identifier: | 74762700 |
| NCBI Gene ID: | 30849 |
| NCBI Accession: | Q99570.3 |
| UniProt Secondary Accession: | Q99570,Q2TBF4, |
| UniProt Related Accession: | Q99570 |
| Molecular Weight: | 1358 |
| NCBI Full Name: | Phosphoinositide 3-kinase regulatory subunit 4 |
| NCBI Synonym Full Names: | phosphoinositide-3-kinase, regulatory subunit 4 |
| NCBI Official Symbol: | PIK3R4 |
| NCBI Official Synonym Symbols: | p150; VPS15 |
| NCBI Protein Information: | phosphoinositide 3-kinase regulatory subunit 4; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphoinositide 3-kinase adaptor protein; phosphatidylinositol 3-kinase-associated p150; phosphoinositide-3-kinase, regulatory subunit 4, p150 |
| UniProt Protein Name: | Phosphoinositide 3-kinase regulatory subunit 4 |
| UniProt Synonym Protein Names: | PI3-kinase p150 subunit; Phosphoinositide 3-kinase adaptor protein |
| Protein Family: | Phosphoinositide 3-kinase regulatory |
| UniProt Gene Name: | PIK3R4 |
| UniProt Entry Name: | PI3R4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PIK3R4 antibody (CAB15828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of NIH-3T3 cells using PIK3R4 Polyclonal Antibody (CAB15828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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