Antibody Name:	Anti-PIK3R2 Antibody
Antibody SKU:	CAB17433
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human PIK3R2

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse kidney, Rat brain

Immunogen:	Recombinant protein of human PIK3R2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5296
Uniprot:	O00459
Cellular Location:
Calculated MW:	81kDa
Observed MW:	85KDa

Synonyms:

MPPH, MPPH1, P85B, p85, p85-BETA, PIK3R2

Background:

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.

UniProt Protein Function:	PIK3R2: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Kinase, lipid; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19q13.2-q13.4 Cellular Component: cytosol; nucleus; phosphoinositide 3-kinase complex Molecular Function:1-phosphatidylinositol-3-kinase activity; 1-phosphatidylinositol-3-kinase regulator activity; protein binding; protein heterodimerization activity; protein phosphatase binding; receptor tyrosine kinase binding Biological Process: blood coagulation; cell glucose homeostasis; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; leukocyte migration; nerve growth factor receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein transport; regulation of autophagy; regulation of phosphoinositide 3-kinase activity; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 1
NCBI Summary:	Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]
UniProt Code:	O00459
NCBI GenInfo Identifier:	317373311
NCBI Gene ID:	5296
NCBI Accession:	O00459.2
UniProt Secondary Accession:	O00459,Q5EAT5, Q9UPH9,
UniProt Related Accession:	O00459
Molecular Weight:	81,545 Da
NCBI Full Name:	Phosphatidylinositol 3-kinase regulatory subunit beta
NCBI Synonym Full Names:	phosphoinositide-3-kinase regulatory subunit 2
NCBI Official Symbol:	PIK3R2
NCBI Official Synonym Symbols:	p85; MPPH; P85B; MPPH1; p85-BETA
NCBI Protein Information:	phosphatidylinositol 3-kinase regulatory subunit beta
UniProt Protein Name:	Phosphatidylinositol 3-kinase regulatory subunit beta
UniProt Synonym Protein Names:	Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta
Protein Family:	Phosphatidylinositol 3-kinase regulatory
UniProt Gene Name:	PIK3R2
UniProt Entry Name:	P85B_HUMAN

	Western blot analysis of extracts of various cell lines, using PIK3R2 Rabbit pAb (CAB17433) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat ovary using PIK3R2 antibody (CAB17433) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using PIK3R2 antibody (CAB17433) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using PIK3R2 antibody (CAB17433) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of NIH/3T3 using PIK3R2 antibody (CAB17433) at dilution of 1:100. Blue: DAPI for nuclear staining.