Name: Anti-PIGZ Antibody (CAB14426)
Brand: Metabolism Antibodies 1
SKU: CAB14426
Availability: OutOfStock

Antibody Name:	Anti-PIGZ Antibody
Antibody SKU:	CAB14426
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 435-579 of human PIGZ (NP_079439.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 435-579 of human PIGZ (NP_079439.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVPG LEYL EQVV HAPV LPST PTHY TLLF THTY MPPR HLLH LPGL GAPV EVVD MGGT EDWA LCQT LKSF TRQP ACQV AGGP WLCR LFVV TPGT TRRA VEKC SFPF KNET LLFP HLTL EDPP ALSS LLSG AWRD HLSL HIVE LGEE T
Gene ID:	80235
Uniprot:	Q86VD9
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	63kDa
Observed MW:	63kDa

Synonyms:

PIGZ, GPI-MT-IV, PIG-Z, SMP3

Background:

The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.

UniProt Protein Function:	PIGZ: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues. Belongs to the glycosyltransferase 22 family. PIGZ subfamily.
UniProt Protein Details:	Protein type:Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 2.4.1.-; Transferase; Membrane protein, multi-pass; Membrane protein, integral; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 3q29 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane Molecular Function:alpha-1,2-mannosyltransferase activity; mannosyltransferase activity Biological Process: GPI anchor biosynthetic process
NCBI Summary:	The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86VD9
NCBI GenInfo Identifier:	1034635710
NCBI Gene ID:	80235
NCBI Accession:	XP_016862732.1
UniProt Secondary Accession:	Q86VD9,Q9H9G6,
UniProt Related Accession:	Q86VD9
Molecular Weight:	63 kDa
NCBI Full Name:	GPI mannosyltransferase 4 isoform X3
NCBI Synonym Full Names:	phosphatidylinositol glycan anchor biosynthesis class Z
NCBI Official Symbol:	PIGZ
NCBI Official Synonym Symbols:	SMP3; PIG-Z; GPI-MT-IV
NCBI Protein Information:	GPI mannosyltransferase 4
UniProt Protein Name:	GPI mannosyltransferase 4
UniProt Synonym Protein Names:	GPI mannosyltransferase IV; GPI-MT-IV; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 homolog; hSMP3
UniProt Gene Name:	PIGZ
UniProt Entry Name:	PIGZ_HUMAN

Western blot analysis of extracts of U-87MG cells, using PIGZ antibody (CAB14426) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Antibodies

PIGZ Antibody (PACO11242)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)