Metabolism Antibodies 1

Anti-PIGT Antibody (CAB12119)

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SKU:
CAB12119
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PIGT Antibody
Antibody SKU:CAB12119
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIGT (NP_057021.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-431, LO2, B cells, Mouse brain, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIGT (NP_057021.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LFRM FSRT LTEP CPLA SESR VYVD ITTY NQDN ETLE VHPP PTTT YQDV ILGT RKTY AIYD LLDT AMIN NSRN LNIQ LKWK RPPE NEAP PVPF LHAQ RYVS GYGL QKGE LSTL LYNT HPYR AFPV LLLD TVPW YLRL YVHT LTIT SKGK ENKP SYIH YQPA QDRL QPHL LEML IQLP ANSV TKVS IQFE RALL KWTE YTPD P
Gene ID:51604
Uniprot:Q969N2
Cellular Location:Endoplasmic reticulum membrane, Single-pass type I membrane protein
Calculated MW:41kDa/43kDa/54kDa/57kDa/59kDa/65kDa
Observed MW:66kDa
Synonyms:PIGT, CGI-06, MCAHS3, NDAP, PNH2
Background:This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:PIGT: Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates. Belongs to the PIGT family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: endoplasmic reticulum membrane; GPI-anchor transamidase complex; membrane

Molecular Function:GPI-anchor transamidase activity; protein binding

Biological Process: attachment of GPI anchor to protein

Disease: Multiple Congenital Anomalies-hypotonia-seizures Syndrome 3; Paroxysmal Nocturnal Hemoglobinuria 2

NCBI Summary:This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:Q969N2
NCBI GenInfo Identifier:44888284
NCBI Gene ID:51604
NCBI Accession:Q969N2.1
UniProt Secondary Accession:Q969N2,Q2NL69, Q7Z3N7, Q9BQY7, Q9BQY8, Q9UJG6, Q9Y2Z5 B2RND5, B7Z3N1, B7Z7I8, E1P622, G8JLF5,
UniProt Related Accession:Q969N2
Molecular Weight:66kDa
NCBI Full Name:GPI transamidase component PIG-T
NCBI Synonym Full Names:phosphatidylinositol glycan anchor biosynthesis class T
NCBI Official Symbol:PIGT  
NCBI Official Synonym Symbols:NDAP; PNH2; CGI-06; MCAHS3  
NCBI Protein Information:GPI transamidase component PIG-T
UniProt Protein Name:GPI transamidase component PIG-T
UniProt Synonym Protein Names:Phosphatidylinositol-glycan biosynthesis class T protein
Protein Family:GPI transamidase component
UniProt Gene Name:PIGT  
Anti-PIGT Antibody (CAB12119)Western blot analysis of extracts of various cell lines, using PIGT antibody (CAB12119) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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