Metabolism Antibodies 2

Anti-PIGQ Antibody (CAB17044)

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SKU:
CAB17044
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PIGQ Antibody
Antibody SKU:CAB17044
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIGQ (NP_683721.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIGQ (NP_683721.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVLK AFFP TCCV STDS GLLV GRWV PEQS SAVV LAVL HFPF IPIQ VKQL LAQV RQAS QVGV AVLG TWCH CRQE PEES LGRF LESL GAVF PHEP WLRL CRER GGTF WSCE ATHR QAPT APGA PGED QVML IFYD QRQV LLSQ LHLP TVLP DRQA GATT ASTG GLAA VFDT VARS EVLF RSDR FDEG PVRL SHWQ SEGV EASI
Gene ID:9091
Uniprot:Q9BRB3
Cellular Location:
Calculated MW:
Observed MW:Refer to figures
Synonyms:PIGQ, GPI1, c407A10.1
Background:This gene is involved in the first step in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a N-acetylglucosaminyl transferase component that is part of the complex that catalyzes transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PIGQ: Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Belongs to the PIGQ family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.4.1.198; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex

Molecular Function:phosphatidylinositol N-acetylglucosaminyltransferase activity

Biological Process: carbohydrate metabolic process; preassembly of GPI anchor in ER membrane

NCBI Summary:This gene is involved in the first step in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a N-acetylglucosaminyl transferase component that is part of the complex that catalyzes transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
UniProt Code:Q9BRB3
NCBI GenInfo Identifier:22538450
NCBI Gene ID:9091
NCBI Accession:NP_004195.2
UniProt Secondary Accession:Q9BRB3,O14927, Q96G00, Q96S22, Q9UJH4, A2IDE1, D3DU52
UniProt Related Accession:Q9BRB3
Molecular Weight:32,471 Da
NCBI Full Name:phosphatidylinositol N-acetylglucosaminyltransferase subunit Q isoform 2
NCBI Synonym Full Names:phosphatidylinositol glycan anchor biosynthesis class Q
NCBI Official Symbol:PIGQ  
NCBI Official Synonym Symbols:GPI1; c407A10.1  
NCBI Protein Information:phosphatidylinositol N-acetylglucosaminyltransferase subunit Q
UniProt Protein Name:Phosphatidylinositol N-acetylglucosaminyltransferase subunit Q
UniProt Synonym Protein Names:N-acetylglucosamyl transferase component GPI1; Phosphatidylinositol-glycan biosynthesis class Q protein; PIG-Q
Protein Family:Phosphatidylinositol N-acetylglucosaminyltransferase
UniProt Gene Name:PIGQ  
UniProt Entry Name:PIGQ_HUMAN
Anti-PIGQ Antibody (CAB17044)Western blot analysis of extracts of various cell lines, using PIGQ antibody (CAB17044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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