Description
| Antibody Name: | Anti-PIF4 Antibody (CAB20725) |
| Antibody SKU: | CAB20725 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Arabidopsis thaliana |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Arabidopsis thaliana |
| Positive Samples: | seedling, rosette leaf |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IDYL KSLQ LQLQ VMWM GSGM AAAA ASAP MMFP GVQP QQFI RQIQ SPVQ LPRF PVMD QSAI QNNP GLVC QNPV QNQI ISDR FARY IGGF PHMQ AATQ MQPM E |
| Cellular Location: | |
| Calculated MW: | 48kDa |
| Observed MW: | 55KDa |
| Synonyms: | AtPIF4, MFL8.13, MFL8_13, phytochrome interacting factor 4, SRL2 |
| Background: | Isolated as a semidominant mutation defective in red -light responses. Encodes a nuclear localized bHLH protein that interacts with active PhyB protein. Negatively regulates phyB mediated red light responses. Involved in shade avoidance response. Protein abundance is negatively regulated by PhyB. |
 | Western blot analysis of extracts of various cell lines, using PIF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
