Name: Anti-PIEZO1 Antibody (CAB4340)
Brand: Signal Transduction Antibodies 2
SKU: CAB4340
Availability: OutOfStock

Antibody Name:	Anti-PIEZO1 Antibody
Antibody SKU:	CAB4340
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2230-2420 of human PIEZO1 (NP_001136336.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, LO2, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2230-2420 of human PIEZO1 (NP_001136336.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PSII PFTA QAYE ELSR QFDP QPLA MQFI SQYS PEDI VTAQ IEGS SGAL WRIS PPSR AQMK RELY NGTA DITL RFTW NFQR DLAK GGTV EYAN EKHM LALA PNST ARRQ LASL LEGT SDQS VVIP NLFP KYIR APNG PEAN PVKQ LQPN EEAD YLGV RIQL RREQ GAGA TGFL EWWV IELQ ECRT DCNL LPM
Gene ID:	9780
Uniprot:	Q92508
Cellular Location:	Cell membrane, Cell projection, Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein, lamellipodium membrane
Calculated MW:	286kDa
Observed MW:	300kDa

Synonyms:	PIEZO1, DHS, FAM38A, LMPH3, Mib
Background:	The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis.

UniProt Protein Function:	PIEZO1: Component of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. Belongs to the PIEZO family.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 16q24.3 Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; integral to membrane; plasma membrane Molecular Function:mechanically-gated ion channel activity; cation channel activity Biological Process: regulation of membrane potential; detection of mechanical stimulus; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of integrin activation; cation transport Disease: Dehydrated Hereditary Stomatocytosis With Or Without Pseudohyperkalemia And/or Perinatal Edema
NCBI Summary:	Piezos are large transmembrane proteins conserved among various species, all having between 24 and 36 predicted transmembrane domains. 'Piezo' comes from the Greek 'piesi,' meaning 'pressure.' The FAM38A gene encodes PIEZO1, a protein that induces mechanically activated (MA) currents in various cell types (Coste et al., 2010 [PubMed 20813920]).[supplied by OMIM, Nov 2010]
UniProt Code:	Q92508
NCBI GenInfo Identifier:	317373533
NCBI Gene ID:	9780
NCBI Accession:	Q92508.4
UniProt Secondary Accession:	Q92508,Q0KKZ9, A6NHT9, A7E2B7,
UniProt Related Accession:	Q92508
Molecular Weight:	2521
NCBI Full Name:	Piezo-type mechanosensitive ion channel component 1
NCBI Synonym Full Names:	piezo-type mechanosensitive ion channel component 1
NCBI Official Symbol:	PIEZO1
NCBI Official Synonym Symbols:	DHS; Mib; FAM38A
NCBI Protein Information:	piezo-type mechanosensitive ion channel component 1; family with sequence similarity 38, member A; membrane protein induced by beta-amyloid treatment
UniProt Protein Name:	Piezo-type mechanosensitive ion channel component 1
UniProt Synonym Protein Names:	Membrane protein induced by beta-amyloid treatment; Mib; Protein FAM38A
Protein Family:	Piezo-type mechanosensitive ion channel component
UniProt Gene Name:	PIEZO1
UniProt Entry Name:	PIEZ1_HUMAN

Western blot analysis of extracts of various cell lines, using PIEZO1 antibody (CAB4340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human PIEZO1 (Piezo-type mechanosensitive ion channel component 1) ELISA Kit (HUFI04961)

Human Piezo-type mechanosensitive ion channel component 1 (PIEZO1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)