Signal Transduction Antibodies 1

Anti-PID1 Antibody (CAB13220)

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SKU:
CAB13220
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-PID1 Antibody
Antibody SKU:CAB13220
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 124-248 of human PID1 (NP_060403.3).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse brain, Mouse kidney, Mouse liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 124-248 of human PID1 (NP_060403.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:REDV FPAN ALLE IRPF QVWL HHLD HKGE ATVH MDTF QVAR IAYC TADH NVSP NIFA WVYR EIND DLSY QMDC HAVE CESK LEAK KLAH AMME AFRK TFHS MKSD GRIH SNSS SEEV SQEL ESDD G
Gene ID:55022
Uniprot:Q7Z2X4
Cellular Location:Cytoplasm
Calculated MW:19kDa/24kDa/28kDa
Observed MW:27kDa
Synonyms:PID1, HMFN2073, NYGGF4, P-CLI1, PCLI1
Background:
UniProt Protein Function:PCLI1: Increases proliferation of preadipocytes without affecting adipocytic differentiation. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: cytoplasm; nucleoplasm

Molecular Function:protein binding

Biological Process: energy reserve metabolic process; fat cell differentiation; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane potential

UniProt Code:Q7Z2X4
NCBI GenInfo Identifier:25304093
NCBI Gene ID:55022
NCBI Accession:BC040164
UniProt Secondary Accession:Q7Z2X4,Q68CJ2, Q6ZUS3, Q8IXL0, Q9NWP6, B3KU82,
UniProt Related Accession:Q7Z2X4
Molecular Weight:24,829 Da
NCBI Full Name:Homo sapiens phosphotyrosine interaction domain containing 1, mRNA
NCBI Synonym Full Names:phosphotyrosine interaction domain containing 1
NCBI Official Symbol:PID1  
NCBI Official Synonym Symbols:PCLI1; NYGGF4; P-CLI1; HMFN2073  
NCBI Protein Information:PTB-containing, cubilin and LRP1-interacting protein
UniProt Protein Name:PTB-containing, cubilin and LRP1-interacting protein
UniProt Synonym Protein Names:Phosphotyrosine interaction domain-containing protein 1; Protein NYGGF4
Protein Family:PTB-containing, cubilin and LRP1-interacting protein
UniProt Gene Name:PID1  
UniProt Entry Name:PCLI1_HUMAN
Anti-PID1 Antibody (CAB13220)Western blot analysis of extracts of various cell lines, using PID1 antibody (CAB13220) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-PID1 Antibody (CAB13220)Immunoprecipitation analysis of 25ug extracts of Mouse liver cells using 3ug PID1 antibody (CAB13220). Western blot was performed from the immunoprecipitate using PID1 antibody (CAB13220) at a dilition of 1:1000.
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