Epigenetics & Nuclear Signaling Antibodies 3

Anti-PIAS4 Antibody (CAB5322)

(No reviews yet) Write a Review
SKU:
CAB5322
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PIAS4 Antibody
Antibody SKU:CAB5322
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-260 of human PIAS4 (NP_056981.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-260 of human PIAS4 (NP_056981.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELYE TRYA KKNS EPAP QPHR PLDP LTMH STYD RAGA VPRT PLAG PNID YPVL YGKY LNGL GRLP AKTL KPEV RLVK LPFF NMLD ELLK PTEL VPQN NEKL QESP CIFA LTPR QVEL IRNS RELQ PGVK AVQV VLRI CYSD TSCP QEDQ YPPN IAVK VNHS YCSV PGYY PSNK PGVE PKRP CRPI NLTH LMYL SSAT NRIT V
Gene ID:51588
Uniprot:Q8N2W9
Cellular Location:Nucleus, PML body
Calculated MW:56kDa
Observed MW:57kDa
Synonyms:PIAS4, PIAS-gamma, PIASY, Piasg, ZMIZ6
Background:
UniProt Protein Function:PIAS4: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. Belongs to the PIAS family.
UniProt Protein Details:

Protein type:SUMO conjugating system; Transcription, coactivator/corepressor; EC 6.3.2.-; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding; SUMO ligase activity; ubiquitin protein ligase binding

Biological Process: double-strand break repair via nonhomologous end joining; negative regulation of transcription, DNA-dependent; positive regulation of protein sumoylation; protein sumoylation

UniProt Code:Q8N2W9
NCBI GenInfo Identifier:34922831
NCBI Gene ID:51588
NCBI Accession:Q8N2W9.1
UniProt Secondary Accession:Q8N2W9,O75926, Q96G19, Q9UN16,
UniProt Related Accession:Q8N2W9
Molecular Weight:56,504 Da
NCBI Full Name:E3 SUMO-protein ligase PIAS4
NCBI Synonym Full Names:protein inhibitor of activated STAT 4
NCBI Official Symbol:PIAS4  
NCBI Official Synonym Symbols:PIASY; Piasg; ZMIZ6; PIAS-gamma  
NCBI Protein Information:E3 SUMO-protein ligase PIAS4
UniProt Protein Name:E3 SUMO-protein ligase PIAS4
UniProt Synonym Protein Names:PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma
Protein Family:E3 SUMO-protein ligase
UniProt Gene Name:PIAS4  
UniProt Entry Name:PIAS4_HUMAN
Anti-PIAS4 Antibody (CAB5322)Western blot analysis of extracts of mouse testis, using PIAS4 antibody (CAB5322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products