Epigenetics & Nuclear Signaling Antibodies 1
Anti-PIAS3 Antibody (CAB13394)
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- SKU:
- CAB13394
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PIAS3 Antibody |
| Antibody SKU: | CAB13394 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 399-628 of human PIAS3 (NP_006090.2). |
| Application: | IF |
| Recommended Dilution: | IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 399-628 of human PIAS3 (NP_006090.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FMED GSWC PMKP KKEA SEVC PPPG YGLD GLQY SPVQ GGDP SENK KKVE VIDL TIES SSDE EDLP PTKK HCSV TSAA IPAL PGSK GVLT SGHQ PSSV LRSP AMGT LGGD FLSS LPLH EYPP AFPL GADI QGLD LFSF LQTE SQHY GPSV ITSL DEQD ALGH FFQY RGTP SHFL GPLA PTLG SSHC SATP APPP GRVS SIVA PGGA LREG HGGP LPSG PSLT GCRS DIIS LD |
| Gene ID: | 10401 |
| Uniprot: | Q9Y6X2 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 68kDa |
| Observed MW: |
| Synonyms: | PIAS3, ZMIZ5 |
| Background: | This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. |
| UniProt Protein Function: | PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoplasm; dendrite; synapse; nucleus Molecular Function:protein C-terminus binding; protein binding; potassium channel regulator activity; enzyme binding; zinc ion binding; protein N-terminus binding; SUMO ligase activity; ligase activity Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential |
| NCBI Summary: | This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y6X2 |
| NCBI GenInfo Identifier: | 56405390 |
| NCBI Gene ID: | 10401 |
| NCBI Accession: | Q9Y6X2.2 |
| UniProt Secondary Accession: | Q9Y6X2,Q9UFI3, |
| UniProt Related Accession: | Q9Y6X2 |
| Molecular Weight: | 628 |
| NCBI Full Name: | E3 SUMO-protein ligase PIAS3 |
| NCBI Synonym Full Names: | protein inhibitor of activated STAT, 3 |
| NCBI Official Symbol: | PIAS3 |
| NCBI Official Synonym Symbols: | ZMIZ5 |
| NCBI Protein Information: | E3 SUMO-protein ligase PIAS3; zinc finger, MIZ-type containing 5; protein inhibitor of activated STAT protein 3 |
| UniProt Protein Name: | E3 SUMO-protein ligase PIAS3 |
| UniProt Synonym Protein Names: | Protein inhibitor of activated STAT protein 3 |
| Protein Family: | E3 SUMO-protein ligase |
| UniProt Gene Name: | PIAS3 |
| UniProt Entry Name: | PIAS3_HUMAN |
 | Immunofluorescence analysis of U2OS cells using PIAS3 antibody (CAB13394). |
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