Anti-PIAS1+PIAS2 Antibody (CAB9670)
- SKU:
- CAB9670
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- DDXBP1
- Synonyms:
- GBP
- Synonyms:
- GU/RH-II
- Synonyms:
- ZMIZ3
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Product Name: | PIAS1+PIAS2 Rabbit mAb |
| Product Code: | CAB9670 |
| Size: | 20uL, 50uL, 100uL |
| Synonyms: | DDXBP1, GBP, GU/RH-II, ZMIZ3 |
| Applications: | WB, IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human PIAS1+PIAS2 |
| Applications: | WB, IHC |
| Recommended Dilutions: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Mouse brain |
| Immunogen: | A synthesized peptide derived from human PIAS1+PIAS2 |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 8554/ 9063 |
| Uniprot: | O75925/O75928 |
| Calculated MW: | 76kDa |
| Observed MW: | 76kDa |
| UniProt Protein Function: | PIAS1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Expressed in numerous tissues with highest level in testis. Belongs to the PIAS family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 15q Cellular Component: nucleoplasm; nucleus; PML body Molecular Function:enzyme binding; protein binding; SUMO ligase activity; transcription corepressor activity Biological Process: JAK-STAT cascade; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein sumoylation; protein sumoylation; regulation of cell proliferation |
| NCBI Summary: | This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| UniProt Code: | O75925 |
| NCBI GenInfo Identifier: | 20138891 |
| NCBI Gene ID: | 8554 |
| NCBI Accession: | O75925.2 |
| UniProt Secondary Accession: | O75925,Q147X4, Q99751, Q9UN02, B2RB67, B3KSY9, C5J4B4 |
| UniProt Related Accession: | O75925 |
| Molecular Weight: | 36,204 Da |
| NCBI Full Name: | E3 SUMO-protein ligase PIAS1 |
| NCBI Synonym Full Names: | protein inhibitor of activated STAT 1 |
| NCBI Official Symbol: | PIAS1 |
| NCBI Official Synonym Symbols: | GBP; ZMIZ3; DDXBP1; GU/RH-II |
| NCBI Protein Information: | E3 SUMO-protein ligase PIAS1 |
| UniProt Protein Name: | E3 SUMO-protein ligase PIAS1 |
| UniProt Synonym Protein Names: | DEAD/H box-binding protein 1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein |
| Protein Family: | E3 SUMO-protein ligase |
| UniProt Gene Name: | PIAS1 |
| UniProt Entry Name: | PIAS1_HUMAN |
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