Name: Anti-PI4K2A Antibody (CAB16546)
Brand: Cell Biology Antibodies 7
SKU: CAB16546
Availability: OutOfStock

Antibody Name:	Anti-PI4K2A Antibody
Antibody SKU:	CAB16546
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 131-479 of human PI4K2A (NP_060895.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 131-479 of human PI4K2A (NP_060895.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YQGS SGSY FVKD PQGR IIAV FKPK NEEP YGHL NPKW TKWL QKLC CPCC FGRD CLVL NQGY LSEA GASL VDQK LELN IVPR TKVV YLAS ETFN YSAI DRVK SRGK RLAL EKVP KVGQ RFNR IGLP PKVG SFQL FVEG YKDA DYWL RRFE AEPL PENT NRQL LLQF ERLV VLDY IIRN TDRG NDNW LIKY DCPM DSSS SRDT DWVV VKEP VIKV AAID NGLA FPLK HPDS WRAY PFYW AWLP QAKV PFSQ EIKD LILP KISD PNFV KDLE EDLY ELFK KDPG FDRG QFHK QIAV MRGQ ILNL TQAL KDNK SPLH LVQM PPVI VETA RSHQ RSSS ESYT QSFQ SRKP FFSW W
Gene ID:	55361
Uniprot:	Q9BTU6
Cellular Location:	Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Lipid-anchor, Membrane, Membrane raft, Mitochondrion, dendrite, presynaptic cell membrane, synapse, synaptosome, trans-Golgi network membrane
Calculated MW:	54kDa
Observed MW:	54kDa

Synonyms:	PI4K2A, PI4KII, PIK42A
Background:	Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs.

UniProt Protein Function:	PI4K2A: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
UniProt Protein Details:	Protein type:EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q24 Cellular Component: Golgi apparatus; neuron projection; mitochondrion; synaptic vesicle membrane; lysosomal membrane; integral to plasma membrane; dendrite; perikaryon; cytosol; lipid raft; presynaptic membrane; cell soma; membrane; early endosome membrane; intrinsic to membrane; cytoplasmic vesicle; cell junction; endosome Molecular Function:protein binding; 1-phosphatidylinositol 4-kinase activity; magnesium ion binding; ATP binding Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; basophil degranulation; phosphatidylinositol biosynthetic process
NCBI Summary:	Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9BTU6
NCBI GenInfo Identifier:	74752344
NCBI Gene ID:	55361
NCBI Accession:	Q9BTU6.1
UniProt Secondary Accession:	Q9BTU6,Q9NSG8, D3DR59,
UniProt Related Accession:	Q9BTU6
Molecular Weight:	479
NCBI Full Name:	Phosphatidylinositol 4-kinase type 2-alpha
NCBI Synonym Full Names:	phosphatidylinositol 4-kinase type 2 alpha
NCBI Official Symbol:	PI4K2A
NCBI Official Synonym Symbols:	PI4KII; PIK42A
NCBI Protein Information:	phosphatidylinositol 4-kinase type 2-alpha; phosphatidylinositol 4-kinase type II-alpha; phosphatidylinositol 4-kinase type II (PI4KII)
UniProt Protein Name:	Phosphatidylinositol 4-kinase type 2-alpha
UniProt Synonym Protein Names:	Phosphatidylinositol 4-kinase type II-alpha
Protein Family:	Phosphatidylinositol 4-kinase
UniProt Gene Name:	PI4K2A
UniProt Entry Name:	P4K2A_HUMAN

Western blot analysis of extracts of HeLa cells, using PI4K2A antibody (CAB16546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

PI4K2A Antibody (PACO11227)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)