Cell Biology Antibodies 5
Anti-PI15 Antibody (CAB14886)
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- SKU:
- CAB14886
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PI15 Antibody |
| Antibody SKU: | CAB14886 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 61-170 of human PI15 (NP_056970.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse lung, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 61-170 of human PI15 (NP_056970.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YISQ NDMI AILD YHNQ VRGK VFPP AANM EYMV WDEN LAKS AEAW AATC IWDH GPSY LLRF LGQN LSVR TGRY RSIL QLVK PWYD EVKD YAFP YPQD CNPR CPMR CFGP MC |
| Gene ID: | 51050 |
| Uniprot: | O43692 |
| Cellular Location: | Secreted |
| Calculated MW: | 29kDa |
| Observed MW: | 33kDa |
| Synonyms: | PI15, CRISP8, P24TI, P25TI |
| Background: | This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. |
| UniProt Protein Function: | PI15: Serine protease inhibitor which displays weak inhibitory activity against trypsin. Belongs to the CRISP family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 8q21.11 |
| NCBI Summary: | This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43692 |
| NCBI GenInfo Identifier: | 74735410 |
| NCBI Gene ID: | 51050 |
| NCBI Accession: | O43692.1 |
| UniProt Secondary Accession: | O43692,Q68CY1, |
| UniProt Related Accession: | O43692 |
| Molecular Weight: | 29,065 Da |
| NCBI Full Name: | Peptidase inhibitor 15 |
| NCBI Synonym Full Names: | peptidase inhibitor 15 |
| NCBI Official Symbol: | PI15 |
| NCBI Official Synonym Symbols: | P24TI; P25TI; CRISP8 |
| NCBI Protein Information: | peptidase inhibitor 15 |
| UniProt Protein Name: | Peptidase inhibitor 15 |
| UniProt Synonym Protein Names: | 25 kDa trypsin inhibitor; p25TI; Cysteine-rich secretory protein 8; CRISP-8; SugarCrisp |
| Protein Family: | Peptidase inhibitor |
| UniProt Gene Name: | PI15 |
| UniProt Entry Name: | PI15_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PI15 antibody (CAB14886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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