Cell Biology Antibodies 11
Anti-PHYHD1 Antibody (CAB7208)
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- SKU:
- CAB7208
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PHYHD1 Antibody |
| Antibody SKU: | CAB7208 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human PHYHD1 (NP_001094346.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse spleen, Mouse liver, Mouse kidney, Mouse heart, Mouse skin, Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human PHYHD1 (NP_001094346.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MACL SPSQ LQKF QQDG FLVL EGFL SAEE CVAM QQRI GEIV AEMD VPLH CRTE FSTQ EEEQ LRAQ GSTD YFLS SGDK IRFF FEKG VFDE KGNF LVPP EKSI NKIG HALH AHDP VFKS ITHS FKVQ TLAR SLGL QMPV VVQS MYIF KQPH FGGE VSPH QDAS FLYT EPLG RVLG VWIA VEDA TLEN GCLW FIPG SHTS GVSR RMVR APVG SAPG TSFL GSEP ARDN SLFV PTPV QRGA LVLI HGEV VHKS KQNL SDRS RQAY TFHL MEAS GTTW SPEN WLQP TAEL PFPQ LYT |
| Gene ID: | 254295 |
| Uniprot: | Q5SRE7 |
| Cellular Location: | |
| Calculated MW: | 30kDa/32kDa |
| Observed MW: | 32kDa |
| Synonyms: | PHYHD1 |
| Background: |
| UniProt Protein Function: | PHYHD1: Isoform 1 has alpha-ketoglutarate-dependent dioxygenase activity. Does not show detectable activity towards fatty acid CoA thioesters. Is not expected to be active with phytanoyl CoA. Isoform 2 and isoform 3 probably lack enzyme activity. Belongs to the PhyH family. PHYHD1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.-.-.-; Oxidoreductase Chromosomal Location of Human Ortholog: 9q34.11 Molecular Function:dioxygenase activity; metal ion binding |
| UniProt Code: | Q5SRE7 |
| NCBI GenInfo Identifier: | 166220781 |
| NCBI Gene ID: | 254295 |
| NCBI Accession: | Q5SRE7.2 |
| UniProt Secondary Accession: | Q5SRE7,Q5SRE9, Q5SRF0, Q7Z623, Q7Z7P9, Q96GM4, A6PWN9 A6PWP0, B3KT57, B4E3X8, |
| UniProt Related Accession: | Q5SRE7 |
| Molecular Weight: | 291 |
| NCBI Full Name: | Phytanoyl-CoA dioxygenase domain-containing protein 1 |
| NCBI Synonym Full Names: | phytanoyl-CoA dioxygenase domain containing 1 |
| NCBI Official Symbol: | PHYHD1 |
| NCBI Protein Information: | phytanoyl-CoA dioxygenase domain-containing protein 1 |
| UniProt Protein Name: | Phytanoyl-CoA dioxygenase domain-containing protein 1 |
| Protein Family: | Phytanoyl-CoA dioxygenase domain-containing protein |
| UniProt Gene Name: | PHYHD1 |
| UniProt Entry Name: | PHYD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHYHD1 antibody (CAB7208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of A-549 cells using PHYHD1 antibody (CAB7208). Blue: DAPI for nuclear staining. |
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