Cell Biology Antibodies 2

Anti-PHPT1 Antibody (CAB1127)

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SKU:
CAB1127
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PHPT1 Antibody
Antibody SKU:CAB1127
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human PHPT1 (NP_054891.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, A-549, THP-1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human PHPT1 (NP_054891.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAVA DLAL IPDV DIDS DGVF KYVL IRVH SAPR SGAP AAES KEIV RGYK WAEY HADI YDKV SGDM QKQG CDCE CLGG GRIS HQSQ DKKI HVYG YSMA YGPA QHAI STEK IKAK YPDY EVTW ANDG Y
Gene ID:29085
Uniprot:Q9NRX4
Cellular Location:Cytoplasm
Calculated MW:13kDa
Observed MW:13kDa
Synonyms:PHPT1, CGI-202, HEL-S-132P, HSPC141, PHP, PHP14
Background:This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function:phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation

NCBI Summary:This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Code:Q9NRX4
NCBI GenInfo Identifier:25008934
NCBI Gene ID:29085
NCBI Accession:Q9NRX4.1
UniProt Secondary Accession:Q9NRX4,Q9H0Y3, B1AMX0, B1AMX1,
UniProt Related Accession:Q9NRX4
Molecular Weight:125
NCBI Full Name:14 kDa phosphohistidine phosphatase
NCBI Synonym Full Names:phosphohistidine phosphatase 1
NCBI Official Symbol:PHPT1  
NCBI Official Synonym Symbols:PHP14; CGI-202; HSPC141; HEL-S-132P  
NCBI Protein Information:14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
UniProt Protein Name:14 kDa phosphohistidine phosphatase
UniProt Synonym Protein Names:Phosphohistidine phosphatase 1; Protein janus-A homolog
Protein Family:14 kDa phosphohistidine phosphatase
UniProt Gene Name:PHPT1  
UniProt Entry Name:PHP14_HUMAN
Anti-PHPT1 Antibody (CAB1127)Western blot analysis of extracts of various cell lines, using PHPT1 Rabbit pAb (CAB1127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-PHPT1 Antibody (CAB1127)Immunohistochemistry of paraffin-embedded rat lung using PHPT1 antibody (CAB1127) at dilution of 1:100 (40x lens).
Anti-PHPT1 Antibody (CAB1127)Immunohistochemistry of paraffin-embedded mouse kidney using PHPT1 antibody (CAB1127) at dilution of 1:100 (40x lens).
Anti-PHPT1 Antibody (CAB1127)Immunohistochemistry of paraffin-embedded mouse heart using PHPT1 antibody (CAB1127) at dilution of 1:100 (40x lens).
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