Cell Biology Antibodies 3
Anti-PHOSPHO2 Antibody (CAB12816)
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- SKU:
- CAB12816
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PHOSPHO2 Antibody |
| Antibody SKU: | CAB12816 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PHOSPHO2 (NP_001008489.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, A-549, NCI-H460 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PHOSPHO2 (NP_001008489.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKIL LVFD FDNT IIDD NSDT WIVQ CAPN KKLP IELR DSYR KGFW TEFM GRVF KYLG DKGV REHE MKRA VTSL PFTP GMVE LFNF IRKN KDKF DCII ISDS |
| Gene ID: | 493911 |
| Uniprot: | Q8TCD6 |
| Cellular Location: | |
| Calculated MW: | 27kDa |
| Observed MW: | 27kDa |
| Synonyms: | PHOSPHO2, phosphatase, orphan 2 |
| Background: |
| UniProt Protein Function: | PHOSPHO2: Phosphatase that has high activity toward pyridoxal 5'- phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. Belongs to the HAD-like hydrolase superfamily. PHOSPHO family. |
| UniProt Protein Details: | Protein type:EC 3.1.3.74; Phosphatase Chromosomal Location of Human Ortholog: 2q31.1 Molecular Function:phosphoric monoester hydrolase activity; protein binding |
| UniProt Code: | Q8TCD6 |
| NCBI GenInfo Identifier: | 74730590 |
| NCBI Gene ID: | 493911 |
| NCBI Accession: | Q8TCD6.1 |
| UniProt Secondary Accession: | Q8TCD6,B2RC30, D3DPC7, |
| UniProt Related Accession: | Q8TCD6 |
| Molecular Weight: | 28kDa |
| NCBI Full Name: | Pyridoxal phosphate phosphatase PHOSPHO2 |
| NCBI Synonym Full Names: | phosphatase, orphan 2 |
| NCBI Official Symbol: | PHOSPHO2 |
| NCBI Protein Information: | pyridoxal phosphate phosphatase PHOSPHO2 |
| UniProt Protein Name: | Pyridoxal phosphate phosphatase PHOSPHO2 |
| Protein Family: | Probable phosphatase |
| UniProt Gene Name: | PHOSPHO2 |
 | Western blot analysis of extracts of various cell lines, using PHOSPHO2 antibody (CAB12816) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using PHOSPHO2 Rabbit pAb (CAB12816) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using PHOSPHO2 Rabbit pAb (CAB12816) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using PHOSPHO2 Rabbit pAb (CAB12816) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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