Epigenetics & Nuclear Signaling Antibodies 4
Anti-Phospho-YAP1-S127 Antibody (CABP0489)
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- SKU:
- CABP0489
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Phospho-YAP1-S127 Antibody |
| Antibody SKU: | CABP0489 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AHSS P |
| Gene ID: | 10413 |
| Uniprot: | P46937 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa |
| Observed MW: | 68kDa |
 | Western blot analysis of extracts of NIH3T3 cells, using Phospho-YAP1-S127 antibody (CABP0489) at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37'C for 60 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
| Synonyms: | COB1, YAP, YAP2, YAP65, YKI, YAP1 |
| Background: | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Code: | P46937 |
| NCBI GenInfo Identifier: | 294862479 |
| NCBI Gene ID: | 10413 |
| NCBI Accession: | P46937.2 |
| UniProt Secondary Accession: | P46937,Q7Z574, B4DTY1, B7ZA01, E3WEB5, E3WEB6, E9PRV2 F5H202, K0KQ18, K0KYZ8, K0L195, K0L1G3, |
| UniProt Related Accession: | P46937 |
| Molecular Weight: | 504 |
| NCBI Full Name: | Transcriptional coactivator YAP1 |
| NCBI Synonym Full Names: | Yes-associated protein 1 |
| NCBI Official Symbol: | YAP1 |
| NCBI Official Synonym Symbols: | YAP; YKI; COB1; YAP2; YAP65 |
| NCBI Protein Information: | transcriptional coactivator YAP1; yorkie homolog; protein yorkie homolog; yes-associated protein 2; 65 kDa Yes-associated protein; yes-associated protein YAP65 homolog |
| UniProt Protein Name: | Transcriptional coactivator YAP1 |
| UniProt Synonym Protein Names: | Protein yorkie homolog; Yes-associated protein YAP65 homolog |
| Protein Family: | Transcriptional coactivator |
| UniProt Gene Name: | YAP1 |
| UniProt Entry Name: | YAP1_HUMAN |
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