Cell Biology Antibodies 16
Anti-Phospho-TNK2-Y284 Antibody (CABP0589)
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- SKU:
- CABP0589
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-TNK2-Y284 Antibody |
| Antibody SKU: | CABP0589 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y284 of human TNK2 (NP_005772.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HepG2, HepG2+EGF |
| Immunogen: | A synthetic phosphorylated peptide around Y284 of human TNK2 (NP_005772.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DHYV M |
| Gene ID: | 10188 |
| Uniprot: | Q07912 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endosome, Membrane, Nucleus, Peripheral membrane protein, adherens junction, clathrin-coated pit, clathrin-coated vesicle |
| Calculated MW: | 60kDa/114kDa/119kDa |
| Observed MW: | 180kDa |
 | Western blot analysis of extracts of HepG2 cells, using Phospho-TNK2-Y284 antibody (CABP0589) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
| Synonyms: | TNK2, ACK, ACK-1, ACK1, p21cdc42Hs |
| Background: | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. |
| UniProt Protein Function: | Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential. Two differentially spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Ack family Chromosomal Location of Human Ortholog: 3q29 Cellular Component: clathrin-coated vesicle; coated pit; cytoplasm; endosome; extrinsic to internal side of plasma membrane; membrane; nucleus; plasma membrane Molecular Function:epidermal growth factor receptor binding; GTPase inhibitor activity; identical protein binding; protein binding; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; WW domain binding Biological Process: cell differentiation; cell migration; cell surface receptor linked signal transduction; innate immune response; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell proliferation; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q07912 |
| NCBI GenInfo Identifier: | 229462980 |
| NCBI Gene ID: | 10188 |
| NCBI Accession: | Q07912.3 |
| UniProt Secondary Accession: | Q07912,Q6ZMQ0, Q8N6U7, Q96H59, |
| UniProt Related Accession: | Q07912 |
| Molecular Weight: | 119,349 Da |
| NCBI Full Name: | Activated CDC42 kinase 1 |
| NCBI Synonym Full Names: | tyrosine kinase non receptor 2 |
| NCBI Official Symbol: | TNK2 |
| NCBI Official Synonym Symbols: | ACK; ACK1; ACK-1; p21cdc42Hs |
| NCBI Protein Information: | activated CDC42 kinase 1 |
| UniProt Protein Name: | Activated CDC42 kinase 1 |
| UniProt Synonym Protein Names: | Tyrosine kinase non-receptor protein 2 |
| Protein Family: | Activated CDC42 kinase |
| UniProt Gene Name: | TNK2 |
| UniProt Entry Name: | ACK1_HUMAN |
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