Immunology Antibodies 3

Anti-Phospho-Syk-Y525/526 Antibody (CABP0542)

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SKU:
CABP0542
Product Type:
Antibody
Applications:
WB
Applications:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-Phospho-Syk-Y525/526 Antibody
Antibody SKU:CABP0542
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around Y525 & Y526 of human Syk (NP_003168.2).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:Human
Positive Samples:Jurkat
Immunogen:A synthetic phosphorylated peptide around Y525 & Y526 of human Syk (NP_003168.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NYYK
Gene ID:6850
Uniprot:P43405
Cellular Location:Cell membrane, Cytoplasm, cytosol
Calculated MW:69kDa/72kDa
Observed MW:72kDa
Anti-Phospho-Syk-Y525/526 Antibody (CABP0542)Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y525/526 antibody (CABP0542) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Synonyms:SYK, p72-Syk
Background:This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.
UniProt Protein Details:

Protein type:EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; TK group; Syk family

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; cytoplasm; plasma membrane; nucleus; cytosol; B cell receptor complex

Molecular Function:integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: viral reproduction; transcription factor import into nucleus; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; activation of JNK activity; leukocyte adhesion; B cell receptor signaling pathway; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; inflammatory response; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; adaptive immune response; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; neutrophil activation during immune response; serotonin secretion by platelet; leukocyte activation during immune response; defense response to bacterium; macrophage activation during immune response; positive regulation of B cell differentiation; blood vessel morphogenesis; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; blood coagulation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion

NCBI Summary:This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Code:P43405
NCBI GenInfo Identifier:1174527
NCBI Gene ID:6850
NCBI Accession:P43405.1
UniProt Related Accession:P43405
Molecular Weight:635
NCBI Full Name:Tyrosine-protein kinase SYK
NCBI Synonym Full Names:spleen tyrosine kinase
NCBI Official Symbol:SYK  
NCBI Official Synonym Symbols:p72-Syk  
NCBI Protein Information:tyrosine-protein kinase SYK
UniProt Protein Name:Tyrosine-protein kinase SYK
UniProt Synonym Protein Names:Spleen tyrosine kinase; p72-Syk
Protein Family:Tyrosine-protein kinase
UniProt Gene Name:SYK  
UniProt Entry Name:KSYK_HUMAN
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