Cell Biology Antibodies 16
Anti-Phospho-Syk-Y352/ZAP70-Y319 Antibody (CABP0501)
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- SKU:
- CABP0501
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-Syk-Y352/ZAP70-Y319 Antibody |
| Antibody SKU: | CABP0501 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y352 of human Syk (NP_003168.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Jurkat, Jurkat+Serum |
| Immunogen: | A synthetic phosphorylated peptide around Y352 of human Syk (NP_003168.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SPYA D |
| Gene ID: | 6850/7535 |
| Uniprot: | P43405/P43403 |
| Cellular Location: | |
| Calculated MW: | 69kDa/72kDa/35kDa/55kDa |
| Observed MW: | 69kDa |
 | Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (CABP0501) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
| Synonyms: | SYK/ZAP70 |
| Background: | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
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