Cell Biology Antibodies 16
Anti-Phospho-SRC-Y416 Antibody (CABP0480)
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- SKU:
- CABP0480
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-SRC-Y416 Antibody |
| Antibody SKU: | CABP0480 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2534/3055/3932/4067/6714/7525 |
| Uniprot: | P06241/P08631/P06239/P07948/P12931/P07947 |
| Cellular Location: | Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton |
| Calculated MW: | 59kDa/60kDa |
| Observed MW: | 60kDa |
 | Western blot analysis of extracts of NIH/3T3-Src cells, using Phospho-SRC Family-Y416 antibody (CABP0480). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SRC Family-Y416 pAb (CABP0480) at 1:1000 dilution or SRC antibody ( A18240).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| Synonyms: | ASV, SRC1, THC6, c-SRC, p60-Src, SRC |
| Background: | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
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