Immunology Antibodies 3
Anti-Phospho-SMAD3-T179 Antibody (CABP0554)
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- SKU:
- CABP0554
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-Phospho-SMAD3-T179 Antibody |
| Antibody SKU: | CABP0554 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, PC-12 |
| Immunogen: | A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PETP P |
| Gene ID: | 4088 |
| Uniprot: | P84022 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 25kDa/35kDa/43kDa/48kDa |
| Observed MW: | 48kDa |
 | Western blot analysis of extracts of HeLa cell line, using Phospho-Smad3-T179 antibody (CABP0554). The lane on the right is treated with the antigen-specific peptide. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Western blot analysis of extracts of PC-12 cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb (CABP0554) at 1:1000 dilution.PC-12 cells were treated by TNF-a (20 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human colon using Phospho-Smad3-T179 antibody (CABP0554) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Smad3-T179 antibody (CABP0554) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat liver using Phospho-Smad3-T179 antibody (CABP0554) at dilution of 1:200 (40x lens). |
| Synonyms: | HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, SMAD3 |
| Background: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. |
| UniProt Protein Function: | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| NCBI Summary: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009] |
| UniProt Code: | P84022 |
| NCBI GenInfo Identifier: | 51338669 |
| NCBI Gene ID: | 4088 |
| NCBI Accession: | P84022.1 |
| UniProt Secondary Accession: | P84022,O09064, O09144, O14510, O35273, Q92940, Q93002 A8K4B6, B7Z4Z5, B7Z6M9, B7Z9Q2, F5H383, |
| UniProt Related Accession: | P84022 |
| Molecular Weight: | 25,722 Da |
| NCBI Full Name: | Mothers against decapentaplegic homolog 3 |
| NCBI Synonym Full Names: | SMAD family member 3 |
| NCBI Official Symbol: | SMAD3 |
| NCBI Official Synonym Symbols: | LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436 |
| NCBI Protein Information: | mothers against decapentaplegic homolog 3 |
| UniProt Protein Name: | Mothers against decapentaplegic homolog 3 |
| UniProt Synonym Protein Names: | JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
| Protein Family: | Mothers against decapentaplegic |
| UniProt Gene Name: | SMAD3 |
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