Epigenetics & Nuclear Signaling Antibodies 5
Anti-Phospho-SF3B1-T313 pAb Antibody (CABP0844)
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- SKU:
- CABP0844
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Phospho-SF3B1-T313 Antibody |
| Antibody SKU: | CABP0844 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, PC-12 |
| Immunogen: | A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AETP R |
| Gene ID: | 23451 |
| Uniprot: | O75533 |
| Cellular Location: | Nucleus speckle |
| Calculated MW: | 16kDa/145kDa |
| Observed MW: | 155kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-SF3B1-T313 antibody (CABP0844) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
| Synonyms: | SF3B1, Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155 |
| Background: | This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | SF3B1: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Phosphorylated during the splicing catalytic steps. |
| UniProt Protein Details: | Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 2q33.1 Cellular Component: nucleoplasm; nucleus; snRNP U2; U12-dependent spliceosome Molecular Function:mRNA binding; protein binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of gene expression, epigenetic; spliceosome assembly Disease: Myelodysplastic Syndrome |
| NCBI Summary: | This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75533 |
| NCBI GenInfo Identifier: | 269849656 |
| NCBI Gene ID: | 23451 |
| NCBI Accession: | O75533.3 |
| UniProt Secondary Accession: | O75533,E9PCH3, |
| UniProt Related Accession: | O75533 |
| Molecular Weight: | 16,020 Da |
| NCBI Full Name: | Splicing factor 3B subunit 1 |
| NCBI Synonym Full Names: | splicing factor 3b subunit 1 |
| NCBI Official Symbol: | SF3B1 |
| NCBI Official Synonym Symbols: | MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155 |
| NCBI Protein Information: | splicing factor 3B subunit 1 |
| UniProt Protein Name: | Splicing factor 3B subunit 1 |
| UniProt Synonym Protein Names: | Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155 |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | SF3B1 |
| UniProt Entry Name: | SF3B1_HUMAN |
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