Immunology Antibodies 3
Anti-Phospho-RPS6KA5-S376 pAb Antibody (CABP0800)
- SKU:
- CABP0800
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-Phospho-RPS6KA5-S376 Antibody |
| Antibody SKU: | CABP0800 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S376 of human RPS6KA5 (NP_004746.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around S376 of human RPS6KA5 (NP_004746.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GYSF V |
| Gene ID: | 9252 |
| Uniprot: | O75582 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 61kDa/81kDa/89kDa |
| Observed MW: | 90kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RPS6KA5-S376 antibody (CABP0800) at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin (25ug/mL) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat testis using Phospho-RPS6KA5-S376 antibody (CABP0800) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-RPS6KA5-S376 antibody (CABP0800) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-RPS6KA5-S376 antibody (CABP0800) at dilution of 1:100 (40x lens). |
| Synonyms: | RPS6KA5, MSK1, MSPK1, RLPK |
| Background: | RPS6KA5 (Ribosomal Protein S6 Kinase A5) is a Protein Coding gene. Diseases associated with RPS6KA5 include Septic Myocarditis and Coffin-Lowry Syndrome. Among its related pathways are Adenylate cyclase inhibitory pathway and Nuclear Events (kinase and transcription factor activation). Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is RPS6KA4. |
| UniProt Protein Function: | MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; MSK subfamily Chromosomal Location of Human Ortholog: 14q31-q32.1 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; histone serine kinase activity (H3-S10 specific); magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; axon guidance; epidermal growth factor receptor signaling pathway; histone phosphorylation; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of cytokine production; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway |
| UniProt Code: | O75582 |
| NCBI GenInfo Identifier: | 37999482 |
| NCBI Gene ID: | 9252 |
| NCBI Accession: | O75582.1 |
| UniProt Secondary Accession: | O75582,O95316, Q96AF7, B7Z2Y5, |
| UniProt Related Accession: | O75582 |
| Molecular Weight: | 81,780 Da |
| NCBI Full Name: | Ribosomal protein S6 kinase alpha-5 |
| NCBI Synonym Full Names: | ribosomal protein S6 kinase A5 |
| NCBI Official Symbol: | RPS6KA5 |
| NCBI Official Synonym Symbols: | MSK1; RLPK; MSPK1 |
| NCBI Protein Information: | ribosomal protein S6 kinase alpha-5 |
| UniProt Protein Name: | Ribosomal protein S6 kinase alpha-5 |
| UniProt Synonym Protein Names: | 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL |
| Protein Family: | Ribosomal protein S6 kinase |
| UniProt Gene Name: | RPS6KA5 |
| UniProt Entry Name: | KS6A5_HUMAN |
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