Cell Biology Antibodies 16
Anti-Phospho-PRKAA1-T183/PRKAA2-T172 Antibody (CABP0116)
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- SKU:
- CABP0116
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-PRKAA1-T183/PRKAA2-T172 Antibody |
| Antibody SKU: | CABP0116 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T172 of human AMPKAlpha |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | C2C12 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T172 of human AMPKAlpha |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5562/5563 |
| Uniprot: | Q13131/P54646 |
| Cellular Location: | |
| Calculated MW: | 64kDa/65kDa |
| Observed MW: | 64KDa |
 | Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (CABP0116) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37'C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat liver using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (CABP0116) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (CABP0116) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using Phospho-PRKAA1-T183-PRKAA2-T172 antibody (CABP0116) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKa1-T183/AMPKa2-T172 pAb (CABP0116). Western blot was performed from the immunoprecipitate using Phospho-AMPKa1-T183/AMPKa2-T172 pAb (CABP0116) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37'C for 30 minutes. |
| Synonyms: | AMPKa1/AMPKa2 |
| Background: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
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