Cell Biology Antibodies 15
Anti-Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Antibody (CABP1164)
(No reviews yet)
Write a Review
- SKU:
- CABP1164
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Description
| Antibody Name: | Anti-Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Antibody |
| Antibody SKU: | CABP1164 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around T696 of human PPP1R12A/PPP1R12B/PPP1R12C. |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Jurkat |
| Immunogen: | A synthetic phosphorylated peptide around T696 of human PPP1R12A/PPP1R12B/PPP1R12C. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4659/4660/54776 |
| Uniprot: | O14974/O60237/Q9BZL4 |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 140kDa |
 | Western blot - Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb (CABP1164) |
| Synonyms: | |
| Background: | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
Related Products
