Cell Biology Antibodies 16
Anti-Phospho-PPIG-S376 pAb Antibody (CABP0842)
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- SKU:
- CABP0842
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-PPIG-S376 Antibody |
| Antibody SKU: | CABP0842 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, C2C12, PC-12 |
| Immunogen: | A synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VSSG E |
| Gene ID: | 9360 |
| Uniprot: | Q13427 |
| Cellular Location: | Nucleus matrix, Nucleus speckle |
| Calculated MW: | 40kDa/88kDa |
| Observed MW: | 110kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-PPIG-S376 antibody (CABP0842) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
| Synonyms: | PPIG, CARS-Cyp, CYP, SCAF10, SRCyp |
| Background: | PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing. |
| UniProt Protein Function: | PPIG: an ubiquitous peptidyl-prolyl cis/trans isomerase. Distributed in nuclear speckles, which are rich in splicing factors. Colocalizes with the splicing factor SFRS2. A component of splicing factor complexes that bind the phosphorylated C-terminal domain (CTD) of RNA polymerase II. Interacts with CLK1 and PNN. Two splice variant isoforms of the human protein have been described. |
| UniProt Protein Details: | Protein type:Isomerase; EC 5.2.1.8; Cyclophilin Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nuclear speck; nucleus Molecular Function:protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding Biological Process: protein peptidyl-prolyl isomerization; protein folding; RNA splicing |
| UniProt Code: | Q13427 |
| NCBI GenInfo Identifier: | 229462749 |
| NCBI Gene ID: | 9360 |
| NCBI Accession: | Q13427.2 |
| UniProt Secondary Accession: | Q13427,O00706, Q53R40, Q53SN4, Q96DG9, D3DPC5, D3DPC6 |
| UniProt Related Accession: | Q13427 |
| Molecular Weight: | 754 |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase G |
| NCBI Synonym Full Names: | peptidylprolyl isomerase G (cyclophilin G) |
| NCBI Official Symbol: | PPIG |
| NCBI Official Synonym Symbols: | CYP; SRCyp; SCAF10; CARS-Cyp |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase G; CASP10; SR-cyp; PPIase G; rotamase G; cyclophilin G; SR-cyclophilin; CARS-cyclophilin; Clk-associating RS-cyclophilin; SR-related CTD-associated factor 10; peptidyl-prolyl isomerase G (cyclophilin G) |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase G |
| UniProt Synonym Protein Names: | CASP10; Clk-associating RS-cyclophilin; CARS-Cyp; CARS-cyclophilin; SR-cyclophilin; SR-cyp; SRcyp; Cyclophilin G; Rotamase G |
| Protein Family: | Cyprosin |
| UniProt Gene Name: | PPIG |
| UniProt Entry Name: | PPIG_HUMAN |
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