Metabolism Antibodies 3
Anti-Phospho-PLCG2-Y1217 pAb Antibody (CABP0805)
- SKU:
- CABP0805
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-Phospho-PLCG2-Y1217 Antibody |
| Antibody SKU: | CABP0805 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y1217 of human PLCG2 (NP_002652.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Raji |
| Immunogen: | A synthetic phosphorylated peptide around Y1217 of human PLCG2 (NP_002652.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FLYD T |
| Gene ID: | 5336 |
| Uniprot: | P16885 |
| Cellular Location: | |
| Calculated MW: | 147kDa |
| Observed MW: | 150kDa |
 | Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y1217 antibody (CABP0805) at 1:1000 dilution or PLCG2 antibody (CAB2182). Raji cells were treated by Pervanadate (1 mM) at 37'C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-PLCG2-Y1217 antibody (CABP0805) at dilution of 1:100 (40x lens). |
| Synonyms: | PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2 |
| Background: | The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. |
| UniProt Protein Function: | PLCG2: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. It is a crucial enzyme in transmembrane signaling. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - inositol phosphate; Phospholipase; EC 3.1.4.11; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 16q24.1 Cellular Component: plasma membrane; cytosol Molecular Function:signal transducer activity; protein binding; phospholipase C activity; phosphoinositide phospholipase C activity Biological Process: platelet activation; Wnt receptor signaling pathway; inositol phosphate metabolic process; phospholipid catabolic process; calcium-mediated signaling; response to lipopolysaccharide; T cell receptor signaling pathway; activation of store-operated calcium channel activity; B cell receptor signaling pathway; regulation of gene expression; inositol trisphosphate biosynthetic process; B cell differentiation; release of sequestered calcium ion into cytosol; negative regulation of programmed cell death; phosphatidylinositol biosynthetic process; innate immune response; follicular B cell differentiation; blood coagulation Disease: Autoinflammation, Antibody Deficiency, And Immune Dysregulation, Plcg2-associated; Familial Cold Autoinflammatory Syndrome 3 |
| NCBI Summary: | The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014] |
| UniProt Code: | P16885 |
| NCBI GenInfo Identifier: | 215274231 |
| NCBI Gene ID: | 5336 |
| NCBI Accession: | P16885.4 |
| UniProt Secondary Accession: | P16885,Q3ZTS2, Q59H45, Q969T5, D3DUL3, |
| UniProt Related Accession: | P16885 |
| Molecular Weight: | 1265 |
| NCBI Full Name: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 |
| NCBI Synonym Full Names: | phospholipase C, gamma 2 (phosphatidylinositol-specific) |
| NCBI Official Symbol: | PLCG2 |
| NCBI Official Synonym Symbols: | FCAS3; APLAID; PLC-IV; PLC-gamma-2 |
| NCBI Protein Information: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; phospholipase C-IV; phosphoinositide phospholipase C-gamma-2 |
| UniProt Protein Name: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 |
| UniProt Synonym Protein Names: | Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2 |
| Protein Family: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase |
| UniProt Gene Name: | PLCG2 |
| UniProt Entry Name: | PLCG2_HUMAN |
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