Cell Biology Antibodies 16

Anti-Phospho-PKC (pan) (zeta)-T410 Antibody (CABP0520)

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SKU:
CABP0520
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Phospho-PKC (pan) (zeta)-T410 Antibody
Antibody SKU:CABP0520
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around T410 of human PRKCZ (NP_002735.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:C6
Immunogen:A synthetic phosphorylated peptide around T410 of human PRKCZ (NP_002735.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TSTF C
Gene ID:5578/5579/5580/5581/5582/5583/5584/5588/5590
Uniprot:P17252/P05771/Q05655/Q02156/P05129/P24723/P41743/Q04759/Q05513
Cellular Location:
Calculated MW:46kDa/56-68kDa/74-83kDa
Observed MW:85kDa
Anti-Phospho-PKC (pan) (zeta)-T410 Antibody (CABP0520)Western blot analysis of extracts of C6 cells, using Phospho-PKC (pan) (zeta)-T410 antibody (CABP0520) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCI/PRKCQ/PRKCZ
Background:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
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