Cell Biology Antibodies 16
Anti-Phospho-PI3KP85 alpha/gamma/beta-Y467/Y199/Y464 pAb Antibody (CABP0854)
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- SKU:
- CABP0854
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-PI3KP85 alpha/gamma/beta-Y467/Y199/Y464 Antibody |
| Antibody SKU: | CABP0854 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | A synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RLYE E |
| Gene ID: | 5295/5296/8503 |
| Uniprot: | P27986/O00459/Q92569 |
| Cellular Location: | |
| Calculated MW: | 42-54kDa/81-84kDa |
| Observed MW: | 70kDa/75kDa |
 | Western blot analysis of extracts of 293T cells, using Phospho-PI3KP85a/g/b-Y467/Y199/Y464 antibody (CABP0854) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of 293T cells, using Phospho-PI3KP85a/g/b-Y467/Y199/Y464 antibody (CABP0854) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | PIK3R1/PIK3R2/PIK3R3 |
| Background: | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
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