Cell Biology Antibodies 16
Anti-Phospho-PDGFRb-Y751 Antibody (CABP0493)
- SKU:
- CABP0493
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-PDGFRb-Y751 Antibody |
| Antibody SKU: | CABP0493 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y751 of human PDGFR beta (NP_002600.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around Y751 of human PDGFR beta (NP_002600.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VDYV P |
| Gene ID: | 5159 |
| Uniprot: | P09619 |
| Cellular Location: | Cell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein |
| Calculated MW: | 37kDa/123kDa |
| Observed MW: | 190kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFR beta-Y751 antibody (CABP0493) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
| Synonyms: | CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT, PDGF Receptor beta, PDGFRB, PDGFR beta |
| Background: | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. |
| UniProt Protein Function: | PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. |
| UniProt Protein Details: | Protein type:EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group Chromosomal Location of Human Ortholog: 5q32 Cellular Component: apical plasma membrane; cytoplasm; focal adhesion; intrinsic to plasma membrane; membrane; nucleus; plasma membrane Molecular Function:enzyme binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet activating factor receptor activity; platelet-derived growth factor beta-receptor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor activity; platelet-derived growth factor receptor binding; protein binding; protein kinase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding Biological Process: cardiac myofibril assembly; cell migration; MAPKKK cascade; peptidyl-tyrosine phosphorylation; phosphatidylinositol metabolic process; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phosphoprotein phosphatase activity; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; signal transduction Disease: Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 4; Kosaki Overgrowth Syndrome; Myeloproliferative Disorder, Chronic, With Eosinophilia; Myofibromatosis, Infantile, 1; Premature Aging Syndrome, Penttinen Type |
| NCBI Summary: | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09619 |
| NCBI GenInfo Identifier: | 129890 |
| NCBI Gene ID: | 5159 |
| NCBI Accession: | P09619.1 |
| UniProt Secondary Accession: | P09619,Q8N5L4, B5A957, |
| UniProt Related Accession: | P09619 |
| Molecular Weight: | Calculated: 37kDa/123kDaObserved: 170kDa |
| NCBI Full Name: | Platelet-derived growth factor receptor beta |
| NCBI Synonym Full Names: | platelet derived growth factor receptor beta |
| NCBI Official Symbol: | PDGFRBÂ Â |
| NCBI Official Synonym Symbols: | IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1Â Â |
| NCBI Protein Information: | platelet-derived growth factor receptor beta |
| UniProt Protein Name: | Platelet-derived growth factor receptor beta |
| UniProt Synonym Protein Names: | Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD_antigen: CD140b |
| Protein Family: | Platelet-derived growth factor receptor |
| UniProt Gene Name: | PDGFRBÂ Â |
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