Immunology Antibodies 3
Anti-Phospho-NFKBIA-Y42 Antibody (CABP0420)
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- SKU:
- CABP0420
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-Phospho-NFKBIA-Y42 Antibody |
| Antibody SKU: | CABP0420 |
| Antibody Size: | 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y42 of human I?BAlpha |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y42 of human I?BAlpha |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4792 |
| Uniprot: | P25963 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 35kDa |
| Observed MW: | 40kDa |
 | Western blot analysis of extracts of HeLa cell lines, using Phospho-IkBalpha-Y42 antibody (CABP0420). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
| Synonyms: | IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2 |
| Background: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
| UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P25963 |
| NCBI GenInfo Identifier: | 126682 |
| NCBI Gene ID: | 4792 |
| NCBI Accession: | P25963.1 |
| UniProt Secondary Accession: | P25963,B2R8L6, |
| UniProt Related Accession: | P25963 |
| Molecular Weight: | 35,609 Da |
| NCBI Full Name: | NF-kappa-B inhibitor alpha |
| NCBI Synonym Full Names: | NFKB inhibitor alpha |
| NCBI Official Symbol: | NFKBIA |
| NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBI |
| NCBI Protein Information: | NF-kappa-B inhibitor alpha |
| UniProt Protein Name: | NF-kappa-B inhibitor alpha |
| UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIA |
| UniProt Entry Name: | IKBA_HUMAN |
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