Description
| Antibody Name: | Anti-Phospho-NFKB2-S866 Antibody |
| Antibody SKU: | CABP0418 |
| Antibody Size: | 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S866 of human NF-?B2 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | ovary cancer |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S866 of human NF-?B2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4791 |
| Uniprot: | Q00653 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 47kDa/96kDa |
| Observed MW: | 120kDa |
 | Western blot analysis of extracts of MDA-MB435 cells, using Phospho-NF-kB2-S866 antibody (CABP0418) at dilution. MDA-MB435 cells were treated by TNF-a. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
 | Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-NF-kB2-S866 antibody (CABP0418). |
 | Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-NF-kB2-S866 antibody (CABP0418). |
| Synonyms: | CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52 |
| Background: | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. |
| UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 10q24 Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; transcription coactivator activity; chromatin binding; transcription factor activity Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; follicular dendritic cell differentiation; MyD88-independent toll-like receptor signaling pathway; rhythmic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; germinal center formation; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway Disease: Immunodeficiency, Common Variable, 10 |
| NCBI Summary: | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| UniProt Code: | Q00653 |
| NCBI GenInfo Identifier: | 116242678 |
| NCBI Gene ID: | 4791 |
| NCBI Accession: | Q00653.4 |
| UniProt Secondary Accession: | Q00653,Q04860, Q9BU75, Q9H471, Q9H472, A8K9D9, D3DR83 |
| UniProt Related Accession: | Q00653,AAB23437 |
| Molecular Weight: | 900 |
| NCBI Full Name: | Nuclear factor NF-kappa-B p100 subunit |
| NCBI Synonym Full Names: | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
| NCBI Official Symbol: | NFKB2 |
| NCBI Official Synonym Symbols: | p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2 |
| NCBI Protein Information: | nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; NFKB, p52/p100 subunit; DNA-binding factor KBF2; transcription factor NFKB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p52 subunit; lymphocyte translocation chromosome 10 protein; nuclear factor of Kappa light chain gene enhancer in B cells 2 |
| UniProt Protein Name: | Nuclear factor NF-kappa-B p100 subunit |
| UniProt Synonym Protein Names: | DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10 |
| Protein Family: | Nuclear factor |
| UniProt Gene Name: | NFKB2 |
| UniProt Entry Name: | NFKB2_HUMAN |
