Description
| Antibody Name: | Anti-Phospho-NDRG1-S330 Antibody |
| Antibody SKU: | CABP0807 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S330 of human NDRG1 (NP_001128714.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat |
| Immunogen: | A synthetic phosphorylated peptide around S330 of human NDRG1 (NP_001128714.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TASG S |
| Gene ID: | 10397 |
| Uniprot: | Q92597 |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center |
| Calculated MW: | 33kDa/35kDa/42kDa |
| Observed MW: | 50kDa |
 | Western blot analysis of extracts of Jurkat cells, using Anti-Phospho-NDRG1-S330 pAb (CABP0807) at 1:2000 dilution or NDRG1 antibody (CAB2142).Jurkat cells were treated by Calyculin A (100nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5 |
| Background: | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D). |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Vesicle Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus Molecular Function:protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation Disease: Charcot-marie-tooth Disease, Type 4d |
| NCBI Summary: | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q92597 |
| NCBI GenInfo Identifier: | 6166568 |
| NCBI Gene ID: | 10397 |
| NCBI Accession: | Q92597.1 |
| UniProt Secondary Accession: | Q92597,O15207, Q6IBG2, Q9NYR6, Q9UK29, B3KR80, B7Z446 |
| UniProt Related Accession: | Q92597 |
| Molecular Weight: | 394 |
| NCBI Full Name: | Protein NDRG1 |
| NCBI Synonym Full Names: | N-myc downstream regulated 1 |
| NCBI Official Symbol: | NDRG1Â Â |
| NCBI Official Synonym Symbols: | GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1Â Â |
| NCBI Protein Information: | protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein |
| UniProt Protein Name: | Protein NDRG1 |
| UniProt Synonym Protein Names: | Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42 |
| Protein Family: | Protein |
| UniProt Gene Name: | NDRG1Â Â |
| UniProt Entry Name: | NDRG1_HUMAN |
