Cell Biology Antibodies 16
Anti-Phospho-MYH9-S1943 pAb Antibody (CABP0802)
- SKU:
- CABP0802
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-MYH9-S1943 Antibody |
| Antibody SKU: | CABP0802 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S1943 of human MYH9 (NP_002464.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | A synthetic phosphorylated peptide around S1943 of human MYH9 (NP_002464.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DGSD E |
| Gene ID: | 4627 |
| Uniprot: | P35579 |
| Cellular Location: | Cytoplasm, cell cortex, cytoskeleton |
| Calculated MW: | 159kDa/226kDa |
| Observed MW: | 250kDa |
 | Western blot analysis of extracts of 293T cells, using Phospho-MYH9-S1943 antibody (CABP0802) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
| Synonyms: | MYH9, BDPLT6, DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA, myosin-9 |
| Background: | This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. |
| UniProt Protein Function: | MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motor; Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: actin cytoskeleton; actomyosin; cleavage furrow; contractile ring; cytoplasm; cytosol; focal adhesion; immunological synapse; integrin complex; leading edge; membrane; myosin II complex; nucleus; plasma membrane; protein complex; ruffle; signalosome; stress fiber; uropod Molecular Function:actin binding; actin filament binding; actin-dependent ATPase activity; ADP binding; ATP binding; ATPase activity; microfilament motor activity; protein anchor; protein binding; protein domain specific binding; protein homodimerization activity Biological Process: actin cytoskeleton reorganization; actin filament-based movement; actomyosin structure organization and biogenesis; angiogenesis; blood vessel endothelial cell migration; cytokinesis; membrane protein ectodomain proteolysis; monocyte differentiation; phagocytosis, engulfment; platelet formation; protein transport; regulation of cell shape Disease: Deafness, Autosomal Dominant 17; Epstein Syndrome; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; May-hegglin Anomaly; Sebastian Syndrome |
| NCBI Summary: | This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011] |
| UniProt Code: | P35579 |
| NCBI GenInfo Identifier: | 6166599 |
| NCBI Gene ID: | 4627 |
| NCBI Accession: | P35579.4 |
| UniProt Secondary Accession: | P35579,O60805, Q60FE2, Q86T83, A8K6E4, |
| UniProt Related Accession: | P35579 |
| Molecular Weight: | 159,864 Da |
| NCBI Full Name: | Myosin-9 |
| NCBI Synonym Full Names: | myosin, heavy chain 9, non-muscle |
| NCBI Official Symbol: | MYH9 |
| NCBI Official Synonym Symbols: | MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA |
| NCBI Protein Information: | myosin-9 |
| UniProt Protein Name: | Myosin-9 |
| UniProt Synonym Protein Names: | Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYH9 |
| UniProt Entry Name: | MYH9_HUMAN |
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