Immunology Antibodies 3
Anti-Phospho-MDM2-S260 Antibody (CABP0074)
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- SKU:
- CABP0074
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-Phospho-MDM2-S260 Antibody |
| Antibody SKU: | CABP0074 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S450 of human MDM2 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S450 of human MDM2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4193 |
| Uniprot: | Q00987 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus, nucleoplasm |
| Calculated MW: | 11-14kDa/24-55kDa |
| Observed MW: | 95kDa |
 | Western blot analysis of extracts of 293 cell line, using phospho-MDM2-S260 antibody (CABP0074). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |
| Synonyms: | MDM2, ACTFS, HDMX, hdm2 |
| Background: | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. |
| UniProt Protein Function: | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis. |
| NCBI Summary: | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013] |
| UniProt Code: | Q00987 |
| NCBI GenInfo Identifier: | 266516 |
| NCBI Gene ID: | 4193 |
| NCBI Accession: | Q00987.1 |
| UniProt Secondary Accession: | Q00987,Q13226, Q13297, Q13298, Q13299, Q13300, Q13301 Q53XW0, Q71TW9, Q8WYJ1, A6NL51, A8K2S6, |
| UniProt Related Accession: | Q00987 |
| Molecular Weight: | 55,991 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase Mdm2 |
| NCBI Synonym Full Names: | MDM2 proto-oncogene |
| NCBI Official Symbol: | MDM2 |
| NCBI Official Synonym Symbols: | HDMX; hdm2; ACTFS |
| NCBI Protein Information: | E3 ubiquitin-protein ligase Mdm2 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase Mdm2 |
| UniProt Synonym Protein Names: | Double minute 2 protein; Hdm2; Oncoprotein Mdm2; RING-type E3 ubiquitin transferase Mdm2Curated; p53-binding protein Mdm2 |
| Protein Family: | Mdm2-binding protein |
| UniProt Gene Name: | MDM2 |
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