Cell Death Antibodies 2
Anti-Phospho-MAP3K5-S83 Antibody (CABP0059)
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- SKU:
- CABP0059
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-Phospho-MAP3K5-S83 Antibody |
| Antibody SKU: | CABP0059 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S83 of human MAP3K5 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | K-562 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S83 of human MAP3K5 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4217 |
| Uniprot: | Q99683 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum |
| Calculated MW: | 69kDa/154kDa |
| Observed MW: | 155kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-MAP3K5-S83 antibody (CABP0059) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour, treated by TNF-a (20ng/mL) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| Synonyms: | MAP3K5, ASK1, MAPKKK5, MEKK5 |
| Background: | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. |
| UniProt Protein Function: | ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens. |
| UniProt Protein Details: | Protein type:EC 2.7.11.25; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family Chromosomal Location of Human Ortholog: 6q23.3 Cellular Component: cytosol Molecular Function:ATP binding; magnesium ion binding; MAP kinase kinase kinase activity; protein binding; protein homodimerization activity; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity Biological Process: activation of JNK activity; activation of MAPKK activity; induction of apoptosis by oxidative stress; JNK cascade; MAPKKK cascade; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of JNK activity; positive regulation of JNK cascade; protein amino acid phosphorylation |
| NCBI Summary: | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99683 |
| NCBI GenInfo Identifier: | 6685617 |
| NCBI Gene ID: | 4217 |
| NCBI Accession: | Q99683.1 |
| UniProt Secondary Accession: | Q99683,Q5THN3, Q99461, A6NIA0, B4DGB2, |
| UniProt Related Accession: | Q99683 |
| Molecular Weight: | 69,839 Da |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase 5 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 5 |
| NCBI Official Symbol: | MAP3K5 |
| NCBI Official Synonym Symbols: | ASK1; MEKK5; MAPKKK5 |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 5 |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 5 |
| UniProt Synonym Protein Names: | Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5 |
| Protein Family: | DASH complex |
| UniProt Gene Name: | MAP3K5 |
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